IFO50423 PKR Cell information
Cell type:general cells
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JCRB No.
IFO50423
Cell Name
PKR
Profile
The name PKR derives from Pig Kidney Rough (outline of cell morphology).
Other Name
Animal
pig
Strain
Genus
Sus
Species
scrofa
Sex
Age
Identity
Tissue for Primary Cancer
kidney
Case history
Metastasis
Tissue Metastasized
Genetics
Life Span
infinite
Crisis PDL
Morphology
epithelial-like
Character
susceptible to Vaccinia virus
Classify
Established by
Ito, H.
Registered by
Ito,H.
Regulation for Distribution
Comment
Year
1993
Medium
Eagle's minimal essential medium with 3% fetal bovine serum and 0.3% lactoalbumin hydrolysate
Methods for Passages
trypsin-EDTA
Cell Number on Passage
Race
CO2 Conc.
5 %
Tissue Sampling
kidney
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
LOT Information
Cell No.
IFO50423
Cell Name
PKR
LOT No.
1029
Lot Specification
distribution
Medium
E.MEM with 3%FBS, 0.3% lactoalbumin hydrolysate
Temperature
37 C
Cell Density at Seeding
Methods for Passages
trypsin-EDTA
Doubling Time
Cell Number in Vial (cells/1ml)
3 x 10^5
Viability at cell freezing (%)
88
Antibiotics Used
free
Passage Number
Unknown (6 at IFO)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NP, LD
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.) - culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information