IFO50424 AP-16

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50424 Cell Name   AP-16
Profile   Astrocyte differentiation is induced by TGF-beta, LIF, CNTF and serum The deprivation of EGF induces death of AP-16 cells by apoptosis. Other Name   
Animal   mouse Strain   C3H/He
Genus   Mus Species   musclus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   neural
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   bipolar Character   astrocyte-progenitor cell line
Classify    Established by   Yoshida, T.
Registered by   Yoshida,T. Regulation for Distribution   
Comment    Year   1993
Medium   D.MEM(high glucose) with 5ug/ml insulin,10ng/ml EGF,50ug/mlTf,10ng/ml biotin and 30nM Na-selenite Methods for Passages   0.1% crystalline trypsin-0.1% soybean trypsin inhibitor (Sigma type I-S) ; polylysine-coated culture dish is required
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain, astrocyte-progenitor cell
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5432
Reference
Pubmed id:8391585Establishment of an astrocyte progenitor cell line: induction of glial fibrillary acidic protein and fibronectin by transforming growth factor-beta 1.
Yoshida T,Takeuchi M
J Neurosci Res. 1993 Jun 1;35(2):129-37




LOT Information

Cell No.   IFO50424 Cell Name   
LOT No.   03282005 Lot Specification   distribution
Medium   DMEM (4.5g/L glucose) with 5ug/ml insulin, 10ng/ml EGF, 50ug/ml transferrin, 10ng/ml biotin, and 20nM Na-selenite Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   0.1% crystelline trypsin. Trypsin action was stopped with 0.1% trypsin inhibitor (Sigma type I-S)
Doubling Time   approx. 1.1 days Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   67.3 Antibiotics Used   free
Passage Number   P14 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD>
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50424 Cell Name   AP-16
LOT No.   1152 Lot Specification   distribution
Medium   D.MEM(high glucose) with 5 ug/ml insulin, 10ng/ml EGF, 50 ug/mlTf, 10ng/ml biotin, 30nM Na-selenite Temperature   37 C
Cell Density at Seeding   1.0-1.5 x 10^5 cells/ml Methods for Passages   0.1% crystalline trypsin-soybean trypsin inhibitor
Doubling Time    Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   Unknown(6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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