IFO50426 NIL-SF2

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50426 Cell Name   NIL-SF2
Profile   NIL subline adapted to serum-free medium Other Name   
Animal   hamster, Syrian Strain   Syrian
Genus   Mesocricetus Species   auratus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   NIL subline adapted to serum-free medium
Classify   variant Established by   Yoshida, T.
Registered by   Yoshida,T. Regulation for Distribution   
Comment    Year   1993
Medium   D.MEM(high glucose) with 5ug/ml insulin,50ug/mlTf,10ng/ml biotin and 30nM Na-selenite Methods for Passages   0.1% crystalline trypsin-0.1% soybean trypsin inhibitor (Sigma type I-S)
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryo, whole
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3072
Pubmed id:1168186Surface carbohydrates of hamster fibroblasts. II. Interaction of hamster NIL cell surfaces with Ricinus communis lectin and concanavalin A as revealed by surface galactosyl label.
Gahmberg CG,Hakomori S
J Biol Chem. 1975 Apr 10;250(7):2447-51
Pubmed id:164440Surface carbohydrates of hamster fibroblasts. I. Chemical characterization of surface-labeled glycosphingolipids and aspecific ceramide tetrasaccharide for transformants.
Gahmberg CG,Hakomori S
J Biol Chem. 1975 Apr 10;250(7):2438-46
Pubmed id:6039764Two spontaneously transformed cell lines derived from the same hamster embryo culture.
Diamond L
Int J Cancer. 1967 Mar 15;2(2):143-52

LOT Information

Cell No.   IFO50426 Cell Name   NIL-SF2
LOT No.   807 Lot Specification   seed/distribution
Medium   D.MEM(high glucose) with 5 ug/ml insulin, 50 ug/mlTf, 10ng/ml biotin, 30nM Na-selenite Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.1% crystalline trypsin-soybean trypsin inhibitor
Doubling Time    Cell Number in Vial (cells/1ml)   4 x 10^6
Viability at cell freezing (%)   67 Antibiotics Used   free
Passage Number   P8 after serum-free PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information