IFO50430 CMK-11-5

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50430 Cell Name   CMK-11-5
Profile   This cell line posseses more mature characteristics than CMK-86 cells. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute megakaryoblastic leukemia Metastasis   
Tissue Metastasized    Genetics   from patient with Down's syndrome
Life Span   infinite Crisis PDL   
Morphology   myeloblast-like Character   leukemia, acute megakaryoblastic
Classify   tumor Established by   Sato, T. et al.
Registered by   Sato,T. Regulation for Distribution   
Comment   This cell line is useful for studying megakaryocytopoiesis. Year   1994
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0217
Reference
Pubmed id:1391807Ultrastructural analysis of platelet-like particles from a human megakaryocytic leukemia cell line (CMK 11-5).
Nagano T,Ohga S,Kishimoto Y,Kimura T,Yasunaga K,Adachi M,Ryo R,Sato T
Int J Hematol. 1992 Aug;56(1):67-78

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LOT Information

Cell No.   IFO50430 Cell Name   
LOT No.   12122008 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^4 cells/ml Methods for Passages   Pipetting and dilution. Some cells loosely attach to substratum.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   95.5 Antibiotics Used   free
Passage Number   Unknown (12 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMSO CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50430 Cell Name   CMK-11-5
LOT No.   978 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   70 Antibiotics Used   free
Passage Number   Unknown (12 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50430 Cell Name   CMK-11-5
LOT No.   12082016 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 1.3 x 10^5 cells/mL Methods for Passages   Pipetting and Dilution (Essentially suspension culture, but Some cells loosely attach to substratum.)
Doubling Time   approx. 34 hrs. Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   72.7 Antibiotics Used   free
Passage Number   Unknown (13 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   72.7 Additional information   
Cell No.   IFO50430 Cell Name   CMK-11-5
LOT No.   01312020 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   3.0 - 3.5 x 10^5 cells/mL Methods for Passages   Pipetting and Dilution (Essentially suspension culture, but some cells loosely attach to substratum.)
Doubling Time   approx. 2 days. Cell Number in Vial (cells/1ml)   91
Viability at cell freezing (%)   4.4 x 10^6 Antibiotics Used   free
Passage Number   Unknown (14 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   87 Additional information   
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