IFO50431 KP-N-YN
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50431 | Cell Name | KP-N-YN |
|---|---|---|---|
| Profile | Alpha smooth muscle actin-positive. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | neural | |
| Case history | neuroblastoma | Metastasis | Yes |
| Tissue Metastasized | pararenal lymph node | Genetics | |
| Life Span | infinite | Crisis PDL | |
| Morphology | Character | neuroblastoma, from lymph node, smooth muscle marker-expressing | |
| Classify | tumor | Established by | Sugimoto, T. et al. |
| Registered by | Sugimoto,T. | Regulation for Distribution | |
| Comment | Year | 1994 | |
| Medium | RPMI1640 medium with 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | neuroblastoma |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1329930 | [Analysis of surface membrane antigens, cytoskeletal proteins and N-myc oncogene in pediatric solid malignant tumors, their diagnostic usefulness and relevant problems]. Sugimoto T,Matsumura T,Horii Y,Saida T,Hosoi H,Saida T,Mine H,Sawada T Hum Cell. 1992 Mar;5(1):1-11 |
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LOT Information
| Cell No. | IFO50431 | Cell Name | KP-N-YN |
|---|---|---|---|
| LOT No. | 1124 | Lot Specification | distribution |
| Medium | RPMI 1640, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | 2 x 10^5 cells/ml | Methods for Passages | trypsin-EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | 2.6 x 10^6 | |
| Viability at cell freezing (%) | 92 | Antibiotics Used | free |
| Passage Number | P26 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD (type B), MD, AST |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:10,11 D13S317:8,11 D7S820:11,13 D16S539:12 VWA:14,17 TH01:7,9 AM:X,Y TPOX:8,11 CSF1PO:10,12 |
|
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.)-culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information | Lot# is labeled as R855F1124 on vials. |
| Cell No. | IFO50431 | Cell Name | KP-N-YN |
|---|---|---|---|
| LOT No. | 12182017 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 3 - 4 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 60 hrs. | Cell Number in Vial (cells/1ml) | 1.2 x 10^7 |
| Viability at cell freezing (%) | 80 | Antibiotics Used | free |
| Passage Number | P27 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 80 | Additional information |