IFO50432 KP-N-RT-BM-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50432 Cell Name   KP-N-RT-BM-1
Profile   neuroblastoma, from bone marrow, neuronal and Schwannian differentiation Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   neural
Case history   neuroblastoma Metastasis   Yes
Tissue Metastasized   bone marrow Genetics   
Life Span   infinite Crisis PDL   
Morphology   neuroblast-like Character   neuroblastoma, from bone marrow, neuronal and Schwannian differentiation
Classify   tumor Established by   Sugimoto, T. et al.
Registered by   Sugimoto,T. Regulation for Distribution   
Comment    Year   1994
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   neuroblastoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1339
Reference
Pubmed id:2833349Schwannian cell differentiation of human neuroblastoma cell lines in vitro induced by bromodeoxyuridine.
Sugimoto T,Kato T,Sawada T,Horii Y,Kemshead JT,Hino T,Morioka H,Hosoi H
Cancer Res. 1988 May 1;48(9):2531-7
Pubmed id:2820569Morphological differentiation of human neuroblastoma cell lines by a new synthetic polyprenoic acid (E5166).
Sugimoto T,Sawada T,Matsumura T,Horii Y,Kemshead JT,Suzuki Y,Okada M,Tagaya O,Hino T
Cancer Res. 1987 Oct 15;47(20):5433-8
Pubmed id:3731124Identical expression of cell surface membrane antigens on two parent and eighteen cloned cell lines derived from two different neuroblastoma metastases of the same patient.
Sugimoto T,Sawada T,Matsumura T,Kemshead JT,Ishii T,Horii Y,Morioka H,Morita M,Reynolds CP
Cancer Res. 1986 Sep;46(9):4765-9

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LOT Information

Cell No.   IFO50432 Cell Name   KP-N-RT-BM-1
LOT No.   1119 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   10^5 cells/ml Methods for Passages   trypsin-EDAT
Doubling Time    Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   P77 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD (type B), MD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50432 Cell Name   KP-N-RT-BM-1
LOT No.   01212014 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.8 -1.4 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   88.5 Antibiotics Used   free
Passage Number   P78 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   88.5 Additional information   
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