IFO50433 KP-N-SI9s

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50433 Cell Name   KP-N-SI9s
Profile   Alpha-smooth muscle actin and desmin-positive. Some S-type cells may have the potential to differentiate into cells with smooth muscle phonotypes. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   neural
Case history   neuroblastoma Metastasis   Yes
Tissue Metastasized   axially lymph node Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   neuroblastoma, from lymph node, smooth muscle marker-expressing
Classify   tumor Established by   Sugimoto, T. et al.
Registered by   Sugimoto,T. Regulation for Distribution   
Comment    Year   1995
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   neuroblastoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1340
Reference
Pubmed id:1329930 [Analysis of surface membrane antigens, cytoskeletal proteins and N-myc oncogene in pediatric solid malignant tumors, their diagnostic usefulness and relevant problems].
Sugimoto T,Matsumura T,Horii Y,Saida T,Hosoi H,Saida T,Mine H,Sawada T
Hum Cell. 1992 Mar;5(1):1-11
Pubmed id:2019470 Alpha-smooth-muscle actin and desmin expressions in human neuroblastoma cell lines.
Sugimoto T,Ueyama H,Hosoi H,Inazawa J,Kato T,Kemshead JT,Reynolds CP,Gown AM,Mine H,Sawada T
Int J Cancer. 1991 May 10;48(2):277-83
Pubmed id:2493986 Differential susceptibility of HLA class II antigens induced by gamma-interferon in human neuroblastoma cell lines.
Sugimoto T,Horii Y,Hino T,Kemshead JT,Kuroda H,Sawada T,Morioka H,Imanishi J,Inoko H
Cancer Res. 1989 Apr 1;49(7):1824-8

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LOT Information

Cell No.   IFO50433 Cell Name   KP-N-SI9s
LOT No.   1125 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   2-4 x 10^4 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   P76 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD (type B), MD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:11,14
D7S820:8,11
D16S539:9,13
VWA:17
TH01:6
AM:X,Y
TPOX:8,11
CSF1PO:10
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50433 Cell Name   KP-N-SI9s
LOT No.   12152017 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   4.3 - 7.7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 49 hrs. Cell Number in Vial (cells/1ml)   5.9 x 10^5
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   P77 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   95.2
Additional information   
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