IFO50433 KP-N-SI9s
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50433 | Cell Name | KP-N-SI9s |
|---|---|---|---|
| Profile | Alpha-smooth muscle actin and desmin-positive. Some S-type cells may have the potential to differentiate into cells with smooth muscle phonotypes. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | neural |
| Case history | neuroblastoma | Metastasis | Yes |
| Tissue Metastasized | axially lymph node | Genetics | |
| Life Span | infinite | Crisis PDL | |
| Morphology | Character | neuroblastoma, from lymph node, smooth muscle marker-expressing | |
| Classify | tumor | Established by | Sugimoto, T. et al. |
| Registered by | Sugimoto,T. | Regulation for Distribution | |
| Comment | Year | 1995 | |
| Medium | RPMI1640 medium with 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | neuroblastoma |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1329930 | [Analysis of surface membrane antigens, cytoskeletal proteins and N-myc oncogene in pediatric solid malignant tumors, their diagnostic usefulness and relevant problems]. Sugimoto T,Matsumura T,Horii Y,Saida T,Hosoi H,Saida T,Mine H,Sawada T Hum Cell. 1992 Mar;5(1):1-11 |
| Pubmed id:2019470 | Alpha-smooth-muscle actin and desmin expressions in human neuroblastoma cell lines. Sugimoto T,Ueyama H,Hosoi H,Inazawa J,Kato T,Kemshead JT,Reynolds CP,Gown AM,Mine H,Sawada T Int J Cancer. 1991 May 10;48(2):277-83 |
| Pubmed id:2486615 | [Establishment of a neuroblastoma cell line KP-N-SI (LA) with clonal interconversion]. Matsumura T,Sugimoto T,Horii Y,Kuroda H,Saida T,Hosoi H,Michihata T,Hino T,Kawakatsu H,Sawada T Hum Cell. 1989 Jun;2(2):206-7 |
| Pubmed id:2493986 | Differential susceptibility of HLA class II antigens induced by gamma-interferon in human neuroblastoma cell lines. Sugimoto T,Horii Y,Hino T,Kemshead JT,Kuroda H,Sawada T,Morioka H,Imanishi J,Inoko H Cancer Res. 1989 Apr 1;49(7):1824-8 |
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LOT Information
| Cell No. | IFO50433 | Cell Name | KP-N-SI9s |
|---|---|---|---|
| LOT No. | 1125 | Lot Specification | distribution |
| Medium | RPMI 1640, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | 2-4 x 10^4 cells/ml | Methods for Passages | trypsin-EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1.3 x 10^6 | |
| Viability at cell freezing (%) | 92 | Antibiotics Used | free |
| Passage Number | P76 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD (type B), MD, AST |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:13 D13S317:11,14 D7S820:8,11 D16S539:9,13 VWA:17 TH01:6 AM:X,Y TPOX:8,11 CSF1PO:10 |
|
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.)-culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50433 | Cell Name | KP-N-SI9s |
|---|---|---|---|
| LOT No. | 12152017 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 4.3 - 7.7 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 49 hrs. | Cell Number in Vial (cells/1ml) | 5.9 x 10^5 |
| Viability at cell freezing (%) | 95.2 | Antibiotics Used | free |
| Passage Number | P77 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 95.2 | Additional information |