IFO50436 KS-1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50436 Cell Name   KS-1
Profile   glioblastoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   45
Identity   available Tissue for Primary Cancer   neural
Case history   glioblastoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   glioblastoma
Classify   tumor Established by   Kanno, T. et al.
Registered by   Kanno,T. Regulation for Distribution   
Comment    Year   1994
Medium   Eagle's minimal essential medium with 1.5% glucose and 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1343
Reference
Pubmed id:4334478 [Cytological study of the cell line derived from malignant human glioblastoma multiforme].
Kanno T,Nakazawa T,Kudo T
Shinkei Kenkyu No Shimpo. 1971;15(4):958-67

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LOT Information

Cell No.   IFO50436 Cell Name   KS-1
LOT No.   1170 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% and 1.5% glucose Temperature   37 C
Cell Density at Seeding   5-10 x 10^4 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   Unknown (9 at IFO) PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:8,12
D13S317:11,13
D7S820:11
D16S539:11,12
VWA:14,18
TH01:6,9
AM:X
TPOX:8,11
CSF1PO:10,12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   Lot no. is labeled as R898F1170.
Cell No.   IFO50436 Cell Name   KS-1
LOT No.   02232017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 1.5% glucose and 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.5 - 1.9 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 53 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   89.5 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89.5
Additional information   
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