IFO50444 KNS-81-FD
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50444 | Cell Name | KNS-81-FD |
|---|---|---|---|
| Profile | GFAP-positive human glioma cell line. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 65 |
| Identity | available | Tissue for Primary Cancer | neural |
| Case history | glioma | Metastasis | |
| Tissue Metastasized | Genetics | serum-free adapted subline of KNS-81 (IFO 50359) | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | glioma, derivative of KNS-81 adapted to serum-free medium |
| Classify | variant | Established by | Takeshita,I. |
| Registered by | Takeshita,I. | Regulation for Distribution | |
| Comment | The DNA fingerprint profile confirmed that this cell line derives from KNS-81. | Year | 1994 |
| Medium | 1 : 1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium. Serum-free | Methods for Passages | 100 USP units/ml crystalline trypsin, 100 protease units/ml dispase and 0.02% trypsin in Ca, Mg-free PBS |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | brain |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Images |
|---|
  |
LOT Information
| Cell No. | IFO50444 | Cell Name | |
|---|---|---|---|
| LOT No. | 03092005 | Lot Specification | distribution |
| Medium | 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium. Serum-free. | Temperature | 37 C |
| Cell Density at Seeding | 5 - 10 x 10^4 cells/ml | Methods for Passages | 100 USP units/ml crystelline trypsin, 100 PU/ml dispase and 0.02% EDTA. Trypsin action was stopped with trypsin inhibitor. Split 1:1 |
| Doubling Time | Very slow (more than 1 week). | Cell Number in Vial (cells/1ml) | 7.6 x 10^5 |
| Viability at cell freezing (%) | 92.7 | Antibiotics Used | free |
| Passage Number | P27 after serum-free | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 0.1% methylcellulose, 10% DMSO - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50444 | Cell Name | KNS-81-FD |
|---|---|---|---|
| LOT No. | 948 | Lot Specification | seed/distribution |
| Medium | Ham'sF12 : D.MEM = 1:1 mixture, serum-free | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | 100 USP units/ml crystalline trypsin, 100 protease units/ml dispase & 0.02% EDTA-PBS | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1 x 10^6 | |
| Viability at cell freezing (%) | 92 | Antibiotics Used | free |
| Passage Number | P20 after serum-free | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD(type B), AST |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:9,12 D13S317:9 D7S820:11 D16S539:10,12 VWA:17,18 TH01:7,9 AM:XY TPOX:8,11 CSF1PO:9,12 |
|
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 0.1% methylcellulose - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Images |
|---|
|
|