IFO50472 MEB5
Cell information
Important Notice(s)Cell type:general cells (View Pricing Information)
JCRB No. | IFO50472 | Cell Name | MEB5 |
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Profile | Neural stem cell line established from embryonic 14.5 day mouse cerebrum by E7 oncogene of HPV type 16. Withdrawal of EGF induces apoptosis or differentiation to neurons, astrocytes, and oligodendrocytes. LIF induces differentiation into astrocytes. | Other Name | |
Animal | mouse | Strain | C3H |
Genus | Mus | Species | musclus |
Sex | Age | 14.5day fetus | |
Identity | Tissue for Primary Cancer | neural | |
Case history | Metastasis | ||
Tissue Metastasized | Genetics | immortalized by E7 oncogene of HPV type 16 | |
Life Span | infinite | Crisis PDL | |
Morphology | round-epithelial | Character | CNS stem cell line, HPV type 16 E7-immortalized |
Classify | Established by | Nakagaito, Y. | |
Registered by | Nakagaito,Y. | Regulation for Distribution | |
Comment | Year | 1997 | |
Medium | Dulbecco's modified Eagle's medium (high glucose) with 5 ug/ml insulin,10 ng/ml EGF, 50 ug/ml transferrin ,10 ng/ml biotin and 30 nM Na-selenite | Methods for Passages | Pipetting, or 0.1% crystalline trypsin in Ca, Mg-free PBS, and the action of trypsin is stopped with 0.1% soybean trypsin inhibitor |
Cell Number on Passage | Race | ||
CO2 Conc. | 5 % | Tissue Sampling | brain |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | not tested | Detected RNA Virus | not tested |
Reference | |
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Pubmed id:10773419 | Activin promotes astrocytic differentiation of a multipotent neural stem cell line and an astrocyte progenitor cell line from murine central nervous system. Satoh M,Sugino H,Yoshida T Neurosci Lett. 2000 Apr 28;284(3):143-6 |
Pubmed id:9719419 | Establishment of an epidermal growth factor-dependent, multipotent neural precursor cell line. Nakagaito Y,Satoh M,Kuno H,Iwama T,Takeuchi M,Hakura A,Yoshida T In Vitro Cell Dev Biol Anim. 1998 Jul-Aug;34(7):585-92 |
LOT Information
Cell No. | IFO50472 | Cell Name | |
---|---|---|---|
LOT No. | 09112009 | Lot Specification | distribution |
Medium | D.MEM(high glucose) with 5 ug/ml insulin, 10ng/ml EGF, 50 ug/mlTf, 10ng/ml biotin, 30nM Na-selenite | Temperature | 37 C |
Cell Density at Seeding | Methods for Passages | Simple dilution or 0.1% crystalline trypsin-trypsin inhibitor | |
Doubling Time | Cell Number in Vial (cells/1ml) | uncountable | |
Viability at cell freezing (%) | Antibiotics Used | free | |
Passage Number | P8 | PDL | |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by MD. |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Exoteric Gene | ||
Medium for Freezing | 10% DMSO, 0.1% methylcellulose - culture medium | CO2 Conc. | 5% |
RFLP | Viability immediately after thawing (%) | ||
Additional information |
Cell No. | IFO50472 | Cell Name | MEB5 |
---|---|---|---|
LOT No. | 1057 | Lot Specification | distribution |
Medium | D.MEM(high glucose) with 5 ug/ml insulin, 10ng/ml EGF, 50 ug/mlTf, 10ng/ml biotin, 30nM Na-selenite | Temperature | 37 C |
Cell Density at Seeding | Methods for Passages | dilution or 0.1% crystalline trypsin-trypsin inhibitor (polylysine, FN, LN-coat) | |
Doubling Time | Cell Number in Vial (cells/1ml) | 3 x 10^6 | |
Viability at cell freezing (%) | 78 | Antibiotics Used | free |
Passage Number | P7 | PDL | |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD, PepB |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Exoteric Gene | ||
Medium for Freezing | 10% DMSO, 0.1% methylcellulose - culture medium | CO2 Conc. | |
RFLP | Viability immediately after thawing (%) | ||
Additional information |
Cell No. | IFO50472 | Cell Name | MEB5 |
---|---|---|---|
LOT No. | 10212016 | Lot Specification | distribution |
Medium | Dulbecco's modified Eagle's medium (high glucose) with 5 ug/ml insulin, 10 ng/ml EGF, 50 ug/ml transferrin, 10 ng/ml biotin and 30 nM Na-selenite | Temperature | 37 C |
Cell Density at Seeding | Methods for Passages | Pipetting, or 0.1% crystalline trypsin in Ca, Mg-free PBS, and the action of trypsin is stopped with 0.1% soybean trypsin inhibitor. | |
Doubling Time | Cell Number in Vial (cells/1ml) | Uncountable (aggregates) | |
Viability at cell freezing (%) | NT | Antibiotics Used | free |
Passage Number | P8 | PDL | NT |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | No | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 0.1% methylcellulose - culture medium | CO2 Conc. | 5% |
RFLP | Viability immediately after thawing (%) | NT | |
Additional information |