IFO50473 MEG-01s

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50473 Cell Name   MEG-01s
Profile   This cell line is useful for studying the differentiation and maturation of megakaryocyts. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   56
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   chronic leukemia Metastasis   
Tissue Metastasized    Genetics   suspension culture adapted subline of MEG-01
Life Span   infinite Crisis PDL   
Morphology   round Character   leukemia, chronic megakaryoblastic, MEG-01-derivative
Classify   variant Established by   Ogura, M. et al.
Registered by   Ogura,M. Regulation for Distribution   
Comment    Year   1998
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   Dilution, split ratio 1:4-1:6
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   bone marrow
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3022
Pubmed id:8954139Protein kinase C plays a key role in the cross-talk between intracellular signalings via prostanoid receptors in a megakaryoblastic cell line, MEG-01s.
Watanabe T,Sunaga S,Togo M,Satoh H,Higashihara M,Hashimoto Y,Kurokawa K
Biochim Biophys Acta. 1996 Nov 22;1304(2):161-9
Pubmed id:7539384MEG-01s cells have receptors for and respond to IL-3, IL-6, and SCF.
Kellar KL,Hooper WC,Benson JM
Exp Hematol. 1995 Jun;23(6):557-64
Pubmed id:1718495Characterization of prostaglandin and thromboxane receptors expressed on a megakaryoblastic leukemia cell line, MEG-01s.
Watanabe T,Yatomi Y,Sunaga S,Miki I,Ishii A,Nakao A,Higashihara M,Seyama Y,Ogura M,Saito H
Blood. 1991 Nov 1;78(9):2328-36
Pubmed id:1367286Properties of a cell line (MEG-01SSF) of human megakaryoblastic leukemia cells.
Takeuchi M,Satoh M,Ogura M,Saito H,Takeuchi K
Res Commun Inst Ferment. 1991;(15):22-9
Pubmed id:2166972Enhanced expression of thrombomodulin by intracellular cyclic AMP-increasing agents in two human megakaryoblastic leukemia cell lines.
Ito T,Ogura M,Morishita Y,Takamatsu J,Maruyama I,Yamamoto S,Ogawa K,Saito H
Thromb Res. 1990 Jun 15;58(6):615-24
Pubmed id:2455575Functional and morphological differentiation induction of a human megakaryoblastic leukemia cell line (MEG-01s) by phorbol diesters.
Ogura M,Morishima Y,Okumura M,Hotta T,Takamoto S,Ohno R,Hirabayashi N,Nagura H,Saito H
Blood. 1988 Jul;72(1):49-60
Pubmed id:2998511Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome.
Ogura M,Morishima Y,Ohno R,Kato Y,Hirabayashi N,Nagura H,Saito H
Blood. 1985 Dec;66(6):1384-92


LOT Information

Cell No.   IFO50473 Cell Name   MEG-01s
LOT No.   1169 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   5.3 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (5 at IFO) PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   NP, G6PD (type B), LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information