IFO50477 FS-1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50477 Cell Name   FS-1
Profile   This cell line produces IL-6 and SCF into its supernatant. Although this cell line was reported as immortalized cell line, the ampoules preserved in the cell bank hardly proliferated. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   bone marrow stroma
Case history   myelogenous leukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   bone marrow stromal cell from a myelogenous leukemia patient
Classify    Established by   Kato, H. et al.
Registered by   Kato,H. Regulation for Distribution   
Comment   Although this cell line was reported as immortalized cell line, the ampoules preserved in the cell bank hardly proliferated. Year   1998
Medium   Iscove's modified Dulbecco's medium with 20% heat-inactivated fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage   1.6 x 10^5 cells/25-cm2 flask Race   
CO2 Conc.   5 % Tissue Sampling   bone marrow stroma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2907
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LOT Information

Cell No.   IFO50477 Cell Name   FS-1
LOT No.   921 Lot Specification   seed/distribution
Medium   Iscove's modification of D.MEM, 80% ; heat-inactivaated FBS, 20% Temperature   37 C
Cell Density at Seeding    Methods for Passages   tryspin-EDTA, 1.6 x 10^5 cells/25 cm2 flask
Doubling Time    Cell Number in Vial (cells/1ml)   3 x 10^5
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   Unknown (5 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:8,11
D7S820:10,12
D16S539:11
VWA:16,18
TH01:6,7
AM:XY
TPOX:9,11
CSF1PO:12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   Labeled as S-A R879F921 on vials.
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