IFO50480 NRK-52E
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50480 | Cell Name | NRK-52E |
|---|---|---|---|
| Profile | NRK-52E is a epithelioid clone of NRK (mixed culture of normal rat kidney) cells. (J. Bacteriol., 92, 1133 (1966)). | Other Name | |
| Animal | rat | Strain | Osbone-Mendel |
| Genus | Rattus | Species | norvegicus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | kidney | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | epithelioid clone of NRK cells |
| Classify | Established by | DeLarco, J. E. & Todaro, G. J. | |
| Registered by | Yoshida,T. | Regulation for Distribution | |
| Comment | Year | 1998 | |
| Medium | Dulbecco's modified Eagle's medium with non-essential amino acids and 5% calf serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | kidney |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
LOT Information
| Cell No. | IFO50480 | Cell Name | |
|---|---|---|---|
| LOT No. | 10032008 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with non-essential amino acids and 10% fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | 2 - 3 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | 0.5 - 1 day | Cell Number in Vial (cells/1ml) | 3.3 x 10^6 |
| Viability at cell freezing (%) | 97 | Antibiotics Used | free |
| Passage Number | P21 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as rat by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50480 | Cell Name | |
|---|---|---|---|
| LOT No. | 07152002 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with non-essential amino acids and 5% fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | 2-5 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS |
| Doubling Time | ca. 24 hrs | Cell Number in Vial (cells/1ml) | 3.9 x 10^6 |
| Viability at cell freezing (%) | 91.3 | Antibiotics Used | free |
| Passage Number | P21 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as rat by NP, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS-DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50480 | Cell Name | NRK-52E |
|---|---|---|---|
| LOT No. | 505 | Lot Specification | seed/distribution |
| Medium | D.MEM with NEAA, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | P18 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, LD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50480 | Cell Name | NRK-52E |
|---|---|---|---|
| LOT No. | 09142015 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with non-essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) | Temperature | 37 C |
| Cell Density at Seeding | 0.7 - 1.5 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA |
| Doubling Time | 14 - 29 hrs | Cell Number in Vial (cells/1ml) | 3.8 x 10^6 |
| Viability at cell freezing (%) | 95.9 | Antibiotics Used | free |
| Passage Number | P21 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 95.9 | Additional information |