IFO50482 CCD 841 CoN

Cell information

Important Notice(s)

Problem of IFO50482 CCD CoN, IFO50483 CCD-33Co

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50482 Cell Name   CCD 841 CoN
Profile   Stop providing (For finite cell growth). This cell line is keratin-negative, therefore difinitive evidence for epithelial origin is lacking. The lots preserved in the cell bank hardly proliferate. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   21-week fetus
Identity   available Tissue for Primary Cancer   colon
Case history   normal colon Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   
Morphology   epithelial-like Character   established from normal colonic tissue, epithelial-like
Classify    Established by   
Registered by   Matsutani,E. Regulation for Distribution   
Comment   The lots preserved in the cell bank hardly proliferate. Year   1998
Medium   ACL-4 medium, serum-free Methods for Passages   0.1% crystalline trypsin in Ca, Mg-free PBS, and the action of trypsin is stopped with 0.1% soybean trypsin inhibitor. Coated culture dish is required
Cell Number on Passage   split ratio 1:3-1:6 Race   
CO2 Conc.   5 % Tissue Sampling   colon
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2871
Reference
Pubmed id:3019544Re: Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium.
Gazdar AF,Oie HK
Cancer Res. 1986 Nov;46(11):6011-2

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LOT Information

Cell No.   IFO50482 Cell Name   CCD 841 CoN
LOT No.   832 Lot Specification   seed/distribution
Medium   ALC-4 medium (a serum free medium based on DMEM:HamF12=1:1 and additives) Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.1% crystalline trypsin-soybean trypsin inhibitor, FN & collagen I coat, split ratio 1:3-1:6
Doubling Time    Cell Number in Vial (cells/1ml)   4 x 10^5
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P16 PDL   32
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:11,13
D7S820:11
D16S539:10,11
VWA:14,18
TH01:7,8
AM:X
TPOX:9,10
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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