IFO50489 GB-1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50489 Cell Name   GB-1
Profile   glioma, GFAP, vimentin and fibronectin-positive Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   35
Identity   available Tissue for Primary Cancer   neural
Case history   glioblastoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   glioblastoma, MDR1 and P-glycoprotein-expressing. GFAP, vimentin and fibronectin-positive
Classify   tumor Established by   Morimura, T.
Registered by   Morimura,T. Regulation for Distribution   
Comment    Year   1998
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1227
Reference
Pubmed id:2754458 Prolongation of G1 phase in cultured glioma cells by cis-dichlorodiammineplatinum (II) (CDDP): analysis using bromodeoxyuridine (BrdU)-Hoechst technique.
Morimura T
J Neurooncol. 1989 May;7(1):71-9

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LOT Information

Cell No.   IFO50489 Cell Name   GB-1
LOT No.   01232006 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   0.6 - 1.8 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 35 hrs Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   99.5 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,13
D13S317:11
D7S820:11,12
D16S539:9
VWA:16,17
TH01:6,7
AM:X
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50489 Cell Name   GB-1
LOT No.   1095 Lot Specification   distribution
Medium   D.MEM, 90% ; FCS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   Unknown (16 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50489 Cell Name   GB-1
LOT No.   01192018 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.4 - 1.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 20 hrs. Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   98.7 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   98.7
Additional information   
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