Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50490 Cell Name   MDM2-RNB
Profile   human MDM2 protein-expressing astrocyte cell line, (Wistar rat) Other Name   
Animal   rat Strain   Wistar
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   neural
Case history    Metastasis   
Tissue Metastasized    Genetics   human MDM2 gene-introduced
Life Span   infinite Crisis PDL   
Morphology   fibroblastic Character   human MDM2 gene-introduced neonetal rat astrocyte, MDM2-expressing
Classify    Established by   Kondo, S. and Morimura, T.
Registered by   Morimura,T. Regulation for Distribution   
Comment    Year   1998
Medium   Dulbecco's modified Eagle's medium with 10% heat-inactivated fetal bovine serum. 1.0 mg/ml G418 for selection Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_D272
Pubmed id:8895524The transforming activities of MDM2 in cultured neonatal rat astrocytes.
Kondo S,Morimura T,Barnett GH,Kondo Y,Peterson JW,Kaakaji R,Takeuchi J,Toms SA,Liu J,Werbel B,Barna BP
Oncogene. 1996 Oct 17;13(8):1773-9

LOT Information

Cell No.   IFO50490 Cell Name   MDM2-RNB
LOT No.   1092 Lot Specification   seed/distribution
Medium   D.MEM, 90% ; inact. FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free (for selection, 1.0 mg/ml G418)
Passage Number   Unknown (15 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information