IFO50492 AM-38

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50492 Cell Name   AM-38
Profile   glioblastoma, GFAP and S-100-positive, ACNU(a cancer chemotherapy drug)-sensitive Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   36
Identity   available Tissue for Primary Cancer   neural
Case history   glioblastoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   glioblastoma, GFAP and S-100-positive, ACNU (a cancer chemotherapy drug)-sensitive
Classify   tumor Established by   Izumi, I. et al.
Registered by   Minemura,K. Regulation for Distribution   
Comment    Year   1998
Medium   Eagle's minimal essential medium with 20% heat-inactivated fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1070
Reference
Pubmed id:8605218Quantification of O6-methylguanine-DNA methyltransferase mRNA in human brain tumors.
Mineura K,Watanabe K,Yanagisawa T,Kowada M
Biochim Biophys Acta. 1996 Feb 9;1289(1):105-9
Pubmed id:7947440Establishment of the two glioma cell lines: YH and AM.
Izumi I,Mineura K,Watanabe K,Kowada M
Hum Cell. 1994 Jun;7(2):101-5
Pubmed id:8145054Characterization and chemosensitivity of two cell lines derived from human glioblastomas.
Izumu I,Mineura K,Watanabe K,Kowada M
J Neurooncol. 1993 Aug;17(2):111-21


Research results by users. Click!
Pubmed id:32687679 Epithelioid glioblastoma with microglia features: potential for novel therapy.
Nakagomi N,Sakamoto D,Hirose T,Takagi T,Murase M,Nakagomi T,Yoshimura S,Hirota S
Brain Pathol. 2020 Nov;30(6):1119-1133

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LOT Information

Cell No.   IFO50492 Cell Name   
LOT No.   12082003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 20% fetal bovine serum (FBS lot; GIBCO 3792782S) Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca. 1.6 days Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   89.6 Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50492 Cell Name   AM-38
LOT No.   961 Lot Specification   seed/distribution
Medium   E.MEM, 80% ; inact. FBS, 20% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   3 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   Unknown (4 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:8
D7S820:12
D16S539:11,13
VWA:17
TH01:7
AM:XY
TPOX:11
CSF1PO:11,12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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Cell No.   IFO50492 Cell Name   AM-38
LOT No.   09082014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 20% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 38 hrs Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:8
D7S820:12
D16S539:11,13
VWA:17,18,19
TH01:7
AM:X,Y
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   95 Additional information   Ampule was mislabeled as IFO50592. Correct number is IFO50492.
Cell No.   IFO50492 Cell Name   AM-38
LOT No.   11152018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 20% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   93 Additional information   Essentially AM-38 has been maintained as adherent culture, but the attachment is very weak Some cells will form aggregates and these aggregates may sometimes detach from the surface of culture dish.
Cell No.   IFO50492 Cell Name   AM-38
LOT No.   11242023 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 20% fetal bovine serum (FBS; Cat. # Nichirei 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   approx. 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:8
D7S820:12
D16S539:11,13
VWA:17,18,19
TH01:7
AM:X,Y
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   95 Additional information   Essentially AM-38 has been maintained as adherent culture, but the attachment is very weak. Some cells will form aggregates and these aggregates may sometimes detach from the surface of culture dish.
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