-Detailed Information [IFO50492]-

IFO50492 AM-38

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	IFO50492	Cell Name	AM-38
Profile	glioblastoma, GFAP and S-100-positive, ACNU(a cancer chemotherapy drug)-sensitive	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	36
Identity	available	Tissue for Primary Cancer	neural
Case history	glioblastoma	Metastasis
Tissue Metastasized		Genetics
Life Span	infinite	Crisis PDL
Morphology		Character	glioblastoma, GFAP and S-100-positive, ACNU (a cancer chemotherapy drug)-sensitive
Classify	tumor	Established by	Izumi, I. et al.
Registered by	Minemura,K.	Regulation for Distribution
Comment		Year	1998
Medium	Eagle's minimal essential medium with 20% heat-inactivated fetal bovine serum	Methods for Passages	trypsin-EDTA
Cell Number on Passage		Race
CO2 Conc.	5 %	Tissue Sampling	brain
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1070

Reference
Pubmed id:8605218	Quantification of O6-methylguanine-DNA methyltransferase mRNA in human brain tumors. Mineura K,Watanabe K,Yanagisawa T,Kowada M Biochim Biophys Acta. 1996 Feb 9;1289(1):105-9
Pubmed id:7947440	Establishment of the two glioma cell lines: YH and AM. Izumi I,Mineura K,Watanabe K,Kowada M Hum Cell. 1994 Jun;7(2):101-5
Pubmed id:8145054	Characterization and chemosensitivity of two cell lines derived from human glioblastomas. Izumu I,Mineura K,Watanabe K,Kowada M J Neurooncol. 1993 Aug;17(2):111-21

Research results by users. Click！
Pubmed id:32687679	Epithelioid glioblastoma with microglia features: potential for novel therapy. Nakagomi N,Sakamoto D,Hirose T,Takagi T,Murase M,Nakagomi T,Yoshimura S,Hirota S Brain Pathol. 2020 Nov;30(6):1119-1133

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LOT Information

12082003
961
09082014
11152018
11242023

Cell No.	IFO50492	Cell Name
LOT No.	12082003	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 20% fetal bovine serum (FBS lot; GIBCO 3792782S)	Temperature	37 C
Cell Density at Seeding	1 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	D.T. = ca. 1.6 days	Cell Number in Vial (cells/1ml)	1.9 x 10^6
Viability at cell freezing (%)	89.6	Antibiotics Used	free
Passage Number	P7*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50492	Cell Name	AM-38
LOT No.	961	Lot Specification	seed/distribution
Medium	E.MEM, 80% ; inact. FBS, 20%	Temperature	37 C
Cell Density at Seeding		Methods for Passages	trypsin-EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	3 x 10^6
Viability at cell freezing (%)	96	Antibiotics Used	free
Passage Number	Unknown (4 at IFO)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NP, G6PD(type B), AST
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12,13 D13S317:8 D7S820:12 D16S539:11,13 VWA:17 TH01:7 AM:XY TPOX:11 CSF1PO:11,12
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% serum (final conc.) - culture medium	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Images

Cell No.	IFO50492	Cell Name	AM-38
LOT No.	09082014	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 20% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091).	Temperature	37 C
Cell Density at Seeding	approx. 1 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 38 hrs	Cell Number in Vial (cells/1ml)	2.6 x 10^6
Viability at cell freezing (%)	95	Antibiotics Used	free
Passage Number	Unknown (6 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12,13 D13S317:8 D7S820:12 D16S539:11,13 VWA:17,18,19 TH01:7 AM:X,Y TPOX:11 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	95	Additional information	Ampule was mislabeled as IFO50592. Correct number is IFO50492.

Cell No.	IFO50492	Cell Name	AM-38
LOT No.	11152018	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 20% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	approx. 1 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 32 hrs.	Cell Number in Vial (cells/1ml)	2.9 x 10^6
Viability at cell freezing (%)	96	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	93	Additional information	Essentially AM-38 has been maintained as adherent culture, but the attachment is very weak Some cells will form aggregates and these aggregates may sometimes detach from the surface of culture dish.

Cell No.	IFO50492	Cell Name	AM-38
LOT No.	11242023	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 20% fetal bovine serum (FBS; Cat. # Nichirei 174112, Lot 20M00K)	Temperature	37 C
Cell Density at Seeding	approx. 1.5 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 32 hrs.	Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	99	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12,13 D13S317:8 D7S820:12 D16S539:11,13 VWA:17,18,19 TH01:7 AM:X,Y TPOX:11 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	95	Additional information	Essentially AM-38 has been maintained as adherent culture, but the attachment is very weak. Some cells will form aggregates and these aggregates may sometimes detach from the surface of culture dish.