-Detailed Information [IFO50492]-

IFO50492 AM-38

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	IFO50492	Cell Name	AM-38
Profile	glioblastoma, GFAP and S-100-positive, ACNU(a cancer chemotherapy drug)-sensitive	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	36
Identity	available	Tissue for Primary Cancer	neural
Case history	glioblastoma	Metastasis
Tissue Metastasized		Genetics
Life Span	infinite	Crisis PDL
Morphology		Character	glioblastoma, GFAP and S-100-positive, ACNU (a cancer chemotherapy drug)-sensitive
Classify	tumor	Established by	Izumi, I. et al.
Registered by	Minemura,K.	Regulation for Distribution
Comment		Year	1998
Medium	Eagle's minimal essential medium with 20% heat-inactivated fetal bovine serum	Methods for Passages	trypsin-EDTA
Cell Number on Passage		Race
CO2 Conc.	5 %	Tissue Sampling	brain
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1070

Reference
Pubmed id:8605218	Quantification of O6-methylguanine-DNA methyltransferase mRNA in human brain tumors. Mineura K,Watanabe K,Yanagisawa T,Kowada M Biochim Biophys Acta. 1996 Feb 9;1289(1):105-9
Pubmed id:7947440	Establishment of the two glioma cell lines: YH and AM. Izumi I,Mineura K,Watanabe K,Kowada M Hum Cell. 1994 Jun;7(2):101-5
Pubmed id:8145054	Characterization and chemosensitivity of two cell lines derived from human glioblastomas. Izumu I,Mineura K,Watanabe K,Kowada M J Neurooncol. 1993 Aug;17(2):111-21

Research results by users. Click！
Pubmed id:32687679	Epithelioid glioblastoma with microglia features: potential for novel therapy. Nakagomi N,Sakamoto D,Hirose T,Takagi T,Murase M,Nakagomi T,Yoshimura S,Hirota S Brain Pathol. 2020 Nov;30(6):1119-1133

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LOT Information

12082003
961
09082014
11152018

Cell No.	IFO50492	Cell Name
LOT No.	12082003	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 20% fetal bovine serum (FBS lot; GIBCO 3792782S)	Temperature	37 C
Cell Density at Seeding	1 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	D.T. = ca. 1.6 days	Cell Number in Vial (cells/1ml)	1.9 x 10^6
Viability at cell freezing (%)	89.6	Antibiotics Used	free
Passage Number	P7*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
RFLP		Viability immediately after thawing (%)
Additional information

Cell No.	IFO50492	Cell Name	AM-38
LOT No.	961	Lot Specification	seed/distribution
Medium	E.MEM, 80% ; inact. FBS, 20%	Temperature	37 C
Cell Density at Seeding		Methods for Passages	trypsin-EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	3 x 10^6
Viability at cell freezing (%)	96	Antibiotics Used	free
Passage Number	Unknown (4 at IFO)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NP, G6PD(type B), AST
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12,13 D13S317:8 D7S820:12 D16S539:11,13 VWA:17 TH01:7 AM:XY TPOX:11 CSF1PO:11,12
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% serum (final conc.) - culture medium	CO2 Conc.
RFLP		Viability immediately after thawing (%)
Additional information

Images

Cell No.	IFO50492	Cell Name	AM-38
LOT No.	09082014	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 20% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091).	Temperature	37 C
Cell Density at Seeding	approx. 1 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 38 hrs	Cell Number in Vial (cells/1ml)	2.6 x 10^6
Viability at cell freezing (%)	95	Antibiotics Used	free
Passage Number	Unknown (6 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12,13 D13S317:8 D7S820:12 D16S539:11,13 VWA:17,18,19 TH01:7 AM:X,Y TPOX:11 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
RFLP		Viability immediately after thawing (%)	95
Additional information	Ampule was mislabeled as IFO50592. Correct number is IFO50492.

Cell No.	IFO50492	Cell Name	AM-38
LOT No.	11152018	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 20% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	approx. 1 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 32 hrs.	Cell Number in Vial (cells/1ml)	2.9 x 10^6
Viability at cell freezing (%)	96	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
RFLP		Viability immediately after thawing (%)	93
Additional information	Essentially AM-38 has been maintained as adherent culture, but the attachment is very weak Some cells will form aggregates and these aggregates may sometimes detach from the surface of culture dish.