IFO50495 Neuro-2aTG

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50495 Cell Name   Neuro-2aTG
Profile   This cell line is HPRT-deficient clone from EMS-mutagnenized Neuro-2a cells. HAT-sensitive, 6-thioguanine-registant. Other Name   
Animal   mouse Strain   
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   neural
Case history   neuroblastoma Metastasis   
Tissue Metastasized    Genetics   HPRT-deficient
Life Span   infinite Crisis PDL   
Morphology   neuron-like Character   neuroblastoma, HPRT-deficient Neuro-2a cell line
Classify   mutant Established by   Satoh, M. and Takeuchi, M.
Registered by   Satoh,M. Regulation for Distribution   
Comment    Year   1998
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum. For selection, add 5 ug/ml 6-thioguanine. Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   region of spinal cord
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_F275

LOT Information

Cell No.   IFO50495 Cell Name   Neuro-2aTG
LOT No.   1088 Lot Specification   distribution
Medium   D.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time   22 hr Cell Number in Vial (cells/1ml)   7 x 10^5
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   P10 after subcloning PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, MD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   Lot No. is labeled as R953F1088.