IFO50518 KAI3
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50518 | Cell Name | KAI3 |
|---|---|---|---|
| Profile | EBV-infected NK-like cell line, IL-2 dependent | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 13 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | severe chronic active Epstein-Barr virus infection , hypersensitivity to mosquito bite | Metastasis | No |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | round, mononuclear without azure granule | Character | NK-like cell line from a patient with severe chron ic active EBV infection, IL-2-dependent |
| Classify | tumor | Established by | Tsuge, I. et al. |
| Registered by | Tsuge,I. | Regulation for Distribution | |
| Comment | Year | 1999 | |
| Medium | RPMI1640 medium with 10% heat-inactivated fetal bovine serum and 100 units/ml interleukin-2 | Methods for Passages | dilution, change 1/3 volume of medium twice a week |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | + | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:10193407 | Characterization of Epstein-Barr virus (EBV)-infected natural killer (NK) cell proliferation in patients with severe mosquito allergy; establishment of an IL-2-dependent NK-like cell line. Tsuge I,Morishima T,Morita M,Kimura H,Kuzushima K,Matsuoka H Clin Exp Immunol. 1999 Mar;115(3):385-92 |
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LOT Information
| Cell No. | IFO50518 | Cell Name | |
|---|---|---|---|
| LOT No. | 03082007 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% heat-inactivated fetal bovine serum and 100 units/ml human recombinant interleukin-2 (FBS lot; GIBCO 4218333S) | Temperature | 37 C |
| Cell Density at Seeding | 0.5-1.0 x 10^5 cells/ml | Methods for Passages | Dilution. This cell line is weak for freezing, and after thawing about half of cells die. If the condition of cells is not well, stand the flask to be high cell density. |
| Doubling Time | 3.5 days | Cell Number in Vial (cells/1ml) | 1.7 x 10^6 |
| Viability at cell freezing (%) | 79.6 | Antibiotics Used | free |
| Passage Number | Unknown (8 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP and G6PD, LD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50518 | Cell Name | KAI3 |
|---|---|---|---|
| LOT No. | 1140 | Lot Specification | distribution |
| Medium | RPMI1640, 90% ; heat-inactivated FBS, 10% ; 100 units/ml IL-2 | Temperature | 37 C |
| Cell Density at Seeding | 1.0 x 10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | Cell Number in Vial (cells/1ml) | 3.0 x 10^6 | |
| Viability at cell freezing (%) | 66 | Antibiotics Used | free |
| Passage Number | Unknown (4 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | MD, LD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% serum (final conc.)-culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50518 | Cell Name | KAI3 |
|---|---|---|---|
| LOT No. | 07312015 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012) and 100 units/ml recombinant human interleukin-2 (Pepro Tech; Cat. # 200-02) | Temperature | 37 C |
| Cell Density at Seeding | 1 - 2 x 10^5 cells/mL | Methods for Passages | Dilution, change 1/3 volume of medium twice a week. This cell line is weak for freezing, and after thawing about half of cells die. If the condition of cells is not well, stand the flask to be high cell density. |
| Doubling Time | approx. 76 hrs | Cell Number in Vial (cells/1ml) | 1.3 x 10^6 |
| Viability at cell freezing (%) | 55 | Antibiotics Used | free |
| Passage Number | Unknown (12 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:11 D13S317:8,11 D7S820:10,12 D16S539:9,10 VWA:17,18 TH01:8,9 AM:X,Y TPOX:8,11 CSF1PO:12 |
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| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 55 | Additional information |
| Cell No. | IFO50518 | Cell Name | KAI3 |
|---|---|---|---|
| LOT No. | 05012018 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) and 100units/ml human reconbinant Interleukin-2 | Temperature | 37 C |
| Cell Density at Seeding | 1.0-2.0x10^5 cells/ml | Methods for Passages | Dilution (split 1/4-1/8) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 5.4x10^6 |
| Viability at cell freezing (%) | 87.5 | Antibiotics Used | free |
| Passage Number | p10* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:8,11 D7S820:10,12 D16S539:9,10 VWA:17,18 TH01:8,9 AM:X,Y TPOX:8,11 CSF1PO:12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information |
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| Cell No. | IFO50518 | Cell Name | KAI3 |
|---|---|---|---|
| LOT No. | 08222018 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) and 100units/ml human recombinant Interleukin-2 | Temperature | 37 C |
| Cell Density at Seeding | 1-2x10^5 cells/ml | Methods for Passages | Dilution (split 1/2-1/4) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.28x10^6 |
| Viability at cell freezing (%) | 84.0 | Antibiotics Used | free |
| Passage Number | p11* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:8,11 D7S820:10,12 D16S539:9,10 VWA:17,18 TH01:8,9 AM:X,Y TPOX:8,11 CSF1PO:12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information |
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