IFO50519 A1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50519 Cell Name   A1
Profile   derives from p53-deficient mouse brain, used for preparation of conditioned medium for MG5 cells Other Name   
Animal   mouse Strain   129/Sv,BDF1,C57BL/6J
Genus   Mus Species   musclus
Sex    Age   newborn
Identity    Tissue for Primary Cancer   neural
Case history    Metastasis   
Tissue Metastasized    Genetics   Established from p53(-/-) homozygote mice (strain; 129/SvJ, BDF1, C57BL/6J).
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   p53-deficient cell line from newborn mouse brain, used for preparation of conditioned medium for MG5.
Classify    Established by   Ohsawa, K.
Registered by   Ohsawa,K. Regulation for Distribution   
Comment   This cell line is used for the preparation of conditioned medium for MG5 cells (IFO 50520). Cell type of this cell line has not been identified. Year   1999
Medium   Dulbecco's modified Eagle's medium (high glucose) with 10% fetal bovine serum. Change medium every 3-4 days. Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_U223
Reference
Pubmed id:11500035 Iba1 is an actin-cross-linking protein in macrophages/microglia.
Sasaki Y,Ohsawa K,Kanazawa H,Kohsaka S,Imai Y
Biochem Biophys Res Commun. 2001 Aug 17;286(2):292-7
Pubmed id:11406294 Co-induction of argininosuccinate synthetase, cationic amino acid transporter-2, and nitric oxide synthase in activated murine microglial cells.
Kawahara K,Gotoh T,Oyadomari S,Kajizono M,Kuniyasu A,Ohsawa K,Imai Y,Kohsaka S,Nakayama H,Mori M
Brain Res Mol Brain Res. 2001 Jun 20;90(2):165-73
Pubmed id:10934045 Involvement of Iba1 in membrane ruffling and phagocytosis of macrophages/microglia.
Ohsawa K,Imai Y,Kanazawa H,Sasaki Y,Kohsaka S
J Cell Sci. 2000 Sep;113 ( Pt 17)():3073-84
Pubmed id:9383038 Generation and characterization of a microglial cell line, MG5, derived from a p53-deficient mouse.
Ohsawa K,Imai Y,Nakajima K,Kohsaka S
Glia. 1997 Nov;21(3):285-98

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LOT Information

Cell No.   IFO50519 Cell Name   
LOT No.   11132008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose) with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   7 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. Subcultivate twice a week.
Doubling Time   approx. 18 hrs Cell Number in Vial (cells/1ml)   4.4 x 10^6
Viability at cell freezing (%)   93.2 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50519 Cell Name   A1
LOT No.   1143 Lot Specification   distribution
Medium   D.MEM (high glucose), 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   5-10 x 10^4 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (5 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50519 Cell Name   A1
LOT No.   06142017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose) with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.8 - 1.6 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 23 hrs. Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   91.8 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   91.8
Additional information   
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