IFO50520 MG5

Cell information

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Cultivation of IFO50520 MG5

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50520 Cell Name   MG5
Profile   A microglial cell line from p53-deficient mice. Other Name   
Animal   mouse Strain   129/Sv,BDF1,C57BL/6J
Genus   Mus Species   musclus
Sex    Age   newborn
Identity    Tissue for Primary Cancer   neural
Case history    Metastasis   
Tissue Metastasized    Genetics   Established from p53(-/-) homozygote mice (strain; 129/SvJ, BDF1, C57BL/6J).
Life Span   infinite Crisis PDL   
Morphology   microglia-like Character   microglial cell line from p53-deficient mice
Classify    Established by   Ohsawa, K.
Registered by   Ohsawa,K. Regulation for Distribution   
Comment    Year   1999
Medium   3/7 mixture of DMEM (high glucose) with 10% FBS and A1(IFO 50519)-conditioned medium (CM). The CM is filtered (0.22 um) supernatant of subconfluent A1 culture. Methods for Passages   Use bacteria-grade dish/flask. For passage, wash the culture twice with PBS and detach cells in PBS by vigorous pipetting with Pasteur pipette.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5556
Reference
Pubmed id:11500035 Iba1 is an actin-cross-linking protein in macrophages/microglia.
Sasaki Y,Ohsawa K,Kanazawa H,Kohsaka S,Imai Y
Biochem Biophys Res Commun. 2001 Aug 17;286(2):292-7
Pubmed id:11406294 Co-induction of argininosuccinate synthetase, cationic amino acid transporter-2, and nitric oxide synthase in activated murine microglial cells.
Kawahara K,Gotoh T,Oyadomari S,Kajizono M,Kuniyasu A,Ohsawa K,Imai Y,Kohsaka S,Nakayama H,Mori M
Brain Res Mol Brain Res. 2001 Jun 20;90(2):165-73
Pubmed id:10934045 Involvement of Iba1 in membrane ruffling and phagocytosis of macrophages/microglia.
Ohsawa K,Imai Y,Kanazawa H,Sasaki Y,Kohsaka S
J Cell Sci. 2000 Sep;113 ( Pt 17)():3073-84
Pubmed id:9383038 Generation and characterization of a microglial cell line, MG5, derived from a p53-deficient mouse.
Ohsawa K,Imai Y,Nakajima K,Kohsaka S
Glia. 1997 Nov;21(3):285-98

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LOT Information

Cell No.   IFO50520 Cell Name   MG5
LOT No.   02232011 Lot Specification   distribution
Medium   7:3 mixture of A1-conditioned medium and D.MEM (high glucose) with 10% FBS. [Lot number 02232023 is mislabel. Correct lot number is 02232011.] Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   Cells were treated with ice cold PBS (4C for 5 min) and were harvedted by vigoruous pipetting. Split ratio 1:2. Use non-treated (bacterial grade) flasks.
Doubling Time   approx. 82 hrs. Cell Number in Vial (cells/1ml)   5 x 10^5
Viability at cell freezing (%)   65.4 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   Lot# is misprinted as 02232023 on vials.
Cell No.   IFO50520 Cell Name   
LOT No.   02122009 Lot Specification   distribution
Medium   7:3 mixture of A1-conditioned medium and D.MEM (high glucose) with 10% FBS Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^4 cells/ml Methods for Passages   25 ug/ml pronase in Hanks' BSS for 10 min. , and vigorus pipetting.
Doubling Time   approx. 3 days Cell Number in Vial (cells/1ml)   6.3 x 10^5
Viability at cell freezing (%)   93.7 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50520 Cell Name   
LOT No.   12222008 Lot Specification   distribution
Medium   7:3 mixture of A1-conditioned medium and D.MEM (high glucose) with 10% FBS Temperature   37 C
Cell Density at Seeding   3 x 10^4 cells/ml Methods for Passages   25 ug/ml pronase in Hanks' BSS for 10 min. , and vigorus pipetting.
Doubling Time   approx. 3 days Cell Number in Vial (cells/1ml)   2.5 x 10^5 cells/ml
Viability at cell freezing (%)   88.2 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50520 Cell Name   MG5
LOT No.   1159 Lot Specification   distribution
Medium   7:3 mixture of A1-conditioned medium and D.MEM (high glucose) with 10% FBS Temperature   37 C
Cell Density at Seeding   3 x 10^4 cells/ml Methods for Passages   25 ug/ml pronase in Hanks' BSS for 10 min. , and pipetting.
Doubling Time    Cell Number in Vial (cells/1ml)   8.8 x 10^5
Viability at cell freezing (%)   84 Antibiotics Used   free
Passage Number   Unknown (5 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50520 Cell Name   MG5
LOT No.   07172017 Lot Specification   distribution
Medium   7:3 mixture of A1-conditioned medium and D.MEM (high glucose) with 10% FBS. Temperature   37 C
Cell Density at Seeding   4.3 - 5.9 x 10^5 cells/mL Methods for Passages   In this lot, RepCell (Temperature-responsive cell cutureware (Cell Seed Inc.)) was used, and the cells were detached by placing the dishes at 4 C freezer for 30 min. Alternative method: Use bacteria-grade dish/flask. For passage, wash the culture twice
Doubling Time   20 - 84 hrs. Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   87.7 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   87.7
Additional information   
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