JCRB0161 Kasumi-4

Cell information

Important Notice(s)

On the particles appear in cultures of Kasumi-series cell lines

Cultivation of Kasumi-4

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0161 Cell Name   Kasumi-4
Profile   A novel human leukemia cell line established from a patient with chronic myelogenous leukaemia (CML) in blast crisis. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   6
Identity   available Tissue for Primary Cancer   blood
Case history   chronic myelogenous leukaemia (CML) in blast crisis. Metastasis   
Tissue Metastasized    Genetics   t(9:22:11), overexpression of Evi-1 gene
Life Span   infinite Crisis PDL   
Morphology   myeloblast Character   CD34+, CD33+, CD13+
Classify   tumor Established by   Asou,H.
Registered by   Kyoizumi,S. & Asou,H Regulation for Distribution   free
Comment    Year   2000
Medium   RPMI1640 medium with 10-20% heat inactivated fetal calf serum and 10ng/ml GM-CSF. Methods for Passages   Subculture twice a week at split ratio of 1/2 by simple dilution. Suspension cell culture. The size of cells is small, and when you collect the cells, centrifuge at 1500 rpm for 10 min.
Cell Number on Passage   Split ratio = 1/2. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0613
Reference
Pubmed id:8611478 Establishment of a myeloid leukaemia cell line (Kasumi-4) with t(9;22;11)(q34;q11;q13), inv(3)(q21q26) and the EVI1 gene activation from a patient with chronic myelogenous leukaemia in blast crisis.
Asou H,Eguchi M,Suzukawa K,Morishita K,Tanaka K,Date M,Hamamoto K,Kamada N
Br J Haematol. 1996 Apr;93(1):68-74


Research results by users. Click!
Pubmed id:32180206 Kasumi leukemia cell lines: characterization of tumor genomes with ethnic origin and scales of genomic alterations.
Kasai F,Asou H,Ozawa M,Kobayashi K,Kuramitsu H,Satoh M,Kohara A,Kaneko Y,Kawamura M
Hum Cell. 2020 Jul;33(3):868-876

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LOT Information

Cell No.   JCRB0161 Cell Name   Kasumi-4
LOT No.   04282011 Lot Specification   distribution
Medium   RPMI1640 medium with 10ng/ml GM-CSF and 20% heat inactivated fetal bovine serum. Temperature   37 C
Cell Density at Seeding   1.5-5x10^5 cells/ml Methods for Passages   Dilution (split ratio=1/2)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.89x10^6
Viability at cell freezing (%)   83.6 Antibiotics Used   
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:10,12
D7S820:8,10
D16S539:9,13
VWA:16,18
TH01:8,9
AM:X
TPOX:8,11
CSF1PO:10,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB0161 Cell Name   Kasumi-4
LOT No.   01122018 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA12J396) and 10 ng/ml GM-CSF. Temperature   37 C
Cell Density at Seeding   1.4-1.9x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:10,12
D7S820:8,10
D16S539:9,13
VWA:16,18
TH01:8,9
AM:X
TPOX:8,11
CSF1PO:10,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   Cell viability of this cell line is relatively low after freeze-thaw. For successful reestablishment of the cell culture, collect cells from two vials into one tube in a total of 10ml medium. Then, centrifuge cells at higher speed, 1500 rpm for 10 min, than usual. Resuspend the cells in fresh 2ml medium and start cell culture at high cell density using either one 6 cm culture dish or one 25 cm culture flask.
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