JCRB0162 NEC14
Cell information
Important Notice(s)Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0162 | Cell Name | NEC14 |
|---|---|---|---|
| Profile | testicular germ cell tumor | Other Name | NEC14 |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 26 |
| Identity | available | Tissue for Primary Cancer | testis |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | testicular germ cell tumor, 26 year-old, male | |
| Life Span | infinite | Crisis PDL | |
| Morphology | Character | testicular germ cell tumor, 26 year-old, male | |
| Classify | Established by | Yamamoto,T. | |
| Registered by | Motoyama, T. | Regulation for Distribution | |
| Comment | Mycoplasma-eliminated cells by MC-210. The NEC14 is unique out of 219 cell line (04/22/2001). | Year | 1989 |
| Medium | RPMI1640 medium with 10% fetal calf serum. | Methods for Passages | Cells are treated with 0.02 % EDTA and 0.25 % trypsin. |
| Cell Number on Passage | split ratio=1/5 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | testis |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Images |
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LOT Information
| Cell No. | JCRB0162 | Cell Name | |
|---|---|---|---|
| LOT No. | 10182001 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | 1.5 x 10^5 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1.2 x 10^6 | |
| Viability at cell freezing (%) | 78.4 | Antibiotics Used | free |
| Passage Number | P21* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% FBS-RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0162 | Cell Name | NEC14 |
|---|---|---|---|
| LOT No. | 08192014 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) | Temperature | 37 C |
| Cell Density at Seeding | Split ratio = 1:3 | Methods for Passages | pipetting and dilution. This cell line grows with forming aggregates. |
| Doubling Time | slow | Cell Number in Vial (cells/1ml) | 3.2 x 10^5 ? (aggregates) |
| Viability at cell freezing (%) | 71.9 | Antibiotics Used | free |
| Passage Number | Unknown (26 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:13 D13S317:11 D7S820:10,12 D16S539:9,11 VWA:14,17 TH01:8 AM:X,Y TPOX:11 CSF1PO:12 |
|
| Adhesion | bot | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 3.2 x 10^5 ? (a | Additional information |
| Cell No. | JCRB0162 | Cell Name | NEC14 |
|---|---|---|---|
| LOT No. | 03272020 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | split ratio = 1/5 (cell number is hard to count) | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | Cell Number in Vial (cells/1ml) | Uncountable (~2 x 10^6) | |
| Viability at cell freezing (%) | ND | Antibiotics Used | free |
| Passage Number | Unknown (21 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 83 | Additional information |