JCRB0165 KHM-10B
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0165 | Cell Name | KHM-10B |
|---|---|---|---|
| Profile | Human Bukitt's lymphoma, ALL-L3 cell line. HLA-DR,CD19 and surface immunoglobulin(mu, lambda) positive. Ig gene rearrangements observed. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 41 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | tumors in tongue with lymphadenopathy. | Metastasis | |
| Tissue Metastasized | Genetics | HLA-DR,CD19 and surface immunoglobulin(mu, lambda) positive. Ig gene rearrangements observed. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | Bukitt's lymphoma, ALL-L3 cell line. |
| Classify | tumor | Established by | Matsuzaki,H. & Takatsuki,K. |
| Registered by | Matsuzaki,H. | Regulation for Distribution | not applied |
| Comment | The KHM-10B is unique among 219 cell line on 04/22/2001 by STR-PCR. | Year | 1997 |
| Medium | RPMI1640 medium with 20% fetal calf serum at begining of the culture. Serum concentration was reduced to 10 % when cells started to grow steadily . | Methods for Passages | Simple dilution. |
| Cell Number on Passage | 10^6 cells/ml | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:8609722 | Establishment of the novel B acute lymphoblastic leukemia (FAB L3) cell line KHM-10B with a 13q34 abnormality and constitutive expression of c-myc and max during cell cycle. Sonoki T,Matsuzaki H,Miyamoto K,Taniwaki M,Yoshino T,Hata H,Yoshida M,Matsuno F,Nagasaki A,Kuribayashi N Leukemia. 1995 Dec;9(12):2093-9 |
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LOT Information
| Cell No. | JCRB0165 | Cell Name | |
|---|---|---|---|
| LOT No. | 12192001 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum. 20% FBS was used during first 4-6 days after thawing. | Temperature | 37 C |
| Cell Density at Seeding | 2-6 x 10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | Cell Number in Vial (cells/1ml) | 4.5 x 10^6 | |
| Viability at cell freezing (%) | 80.2 | Antibiotics Used | free |
| Passage Number | P17* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:11,12 D13S317:8,11 D7S820:10,12 D16S539:9,12 VWA:17,18 TH01:6 AM:X,Y TPOX:8 CSF1PO:11,12 |
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| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS-RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0165 | Cell Name | KHM-10B |
|---|---|---|---|
| LOT No. | 06302023 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) | Temperature | 37 C |
| Cell Density at Seeding | 1.0-2.01x10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 5.08x10^6 |
| Viability at cell freezing (%) | 91.12 | Antibiotics Used | free |
| Passage Number | p16* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:8,11 D7S820:10,12 D16S539:9,12 VWA:17,18 TH01:6 AM:X,Y TPOX:8 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 87 | Additional information | RPMI1640 with 20% heat inactivated fetal bovine serum at the beginning of the culture. Serum concentration was reduced to 10% when cells started to grow steadily. |
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