JCRB0167 RCN-9

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0167 Cell Name   RCN-9
Profile   DMH induced colonic cancer, (F344/DuCrj rat) Other Name   RCN-9
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex   M Age   20-week
Identity    Tissue for Primary Cancer   colon
Case history    Metastasis   
Tissue Metastasized    Genetics   DMH induced colonic cancer. Male F344/DuCrj rat.
Life Span    Crisis PDL   
Morphology    Character   DMH induced colonic cancer. Male F344/DuCrj rat.
Classify    Established by   Inoue,Y. & Kashima,Y
Registered by   Inoue, Y. et al. Regulation for Distribution   
Comment   Low differentiated adenocarcinoma. Year   2001
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   2x10^5 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   colon
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5349
Reference
Pubmed id:1900274A new rat colon cancer cell line metastasizes spontaneously: biologic characteristics and chemotherapeutic response.
Inoue Y,Kashima Y,Aizawa K,Hatakeyama K
Jpn J Cancer Res. 1991 Jan;82(1):90-7


Research results by users. Click！
Pubmed id:9655087 Involvement of liver-associated immunity in hepatic metastasis formation.
Okuno K,Hirai N,Lee YS,Kawai I,Shigeoka H,Yasutomi M
J Surg Res. 1998 Mar;75(2):148-52

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LOT Information

Cell No.   JCRB0167 Cell Name   
LOT No.   06202002 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   1.0 x 10^5 cells/ml or lower. Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   4.1 x 10^6
Viability at cell freezing (%)   95.6 Antibiotics Used   free
Passage Number   P129 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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