JCRB0166 HMC-1-8
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0166 | Cell Name | HMC-1-8 |
|---|---|---|---|
| Profile | A target cell of the T-cell killer clone T(cHMC-1). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 35 |
| Identity | available | Tissue for Primary Cancer | mammary |
| Case history | a person had undergone left radical mastectomy 3 years previously for an infiltrating scirrhous carcinoma of the left breast. | Metastasis | Yes |
| Tissue Metastasized | pleural effusion | Genetics | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | HMC-1-8 is a target cell of the T-cell killer clone T(cHMC-1) derived from breast cancer established from pleural effusion. |
| Classify | tumor | Established by | Kikuchi,H. |
| Registered by | Sato,T. | Regulation for Distribution | not described |
| Comment | Year | 2001 | |
| Medium | RPMI1640 medium with 10% fetal bovine serum. | Methods for Passages | Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA at room temperature for 10 minutes. Subculture once a week and change medium twice a week. |
| Cell Number on Passage | 8 x 10^4 cells/ml | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | pleural effusion |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2786747 | Identification by monoclonal antibody of the tumor antigen of a human autologous breast cancer cell that is involved in cytotoxicity by a cytotoxic T-cell clone. Okubo M,Sato N,Wada Y,Takahashi S,Torimoto K,Takahashi N,Sato T,Okazaki M,Asaishi K,Kikuchi K Cancer Res. 1989 Jul 15;49(14):3950-4 |
| Pubmed id:3488124 | Specific cytotoxicity of a long-term cultured T-cell clone on human autologous mammary cancer cells. Sato T,Sato N,Takahashi S,Koshiba H,Kikuchi K Cancer Res. 1986 Sep;46(9):4384-9 |
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LOT Information
| Cell No. | JCRB0166 | Cell Name | HMC-1-8 |
|---|---|---|---|
| LOT No. | 05222001 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (Intergen RB52405). | Temperature | 37 C |
| Cell Density at Seeding | 8.0 x 10^4 cells/ml | Methods for Passages | Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA at room temp. for 10 min. Subculture once a week and medium change twice a week. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.0 x 10^6 |
| Viability at cell freezing (%) | 96.7 | Antibiotics Used | free |
| Passage Number | P17* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0166 | Cell Name | HMC-1-8 |
|---|---|---|---|
| LOT No. | 12052013 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10091). | Temperature | 37C |
| Cell Density at Seeding | 0.9 - 1.7 x 10^5 cells/mL | Methods for Passages | Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA at room temperature for 10 minutes. Subculture once a week and change medium twice a week. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 4.2 x 10^6 (cells/1 | |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | Unknown (20 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeB),NP,LDH were examined. Human,not HeLa |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | 5% | |
| Viability immediately after thawing (%) | 93.3 | Additional information |
| Cell No. | JCRB0166 | Cell Name | HMC-1-8 |
|---|---|---|---|
| LOT No. | 05022023 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322) | Temperature | 37 C |
| Cell Density at Seeding | 1.6 x -4.0 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 1 day | Cell Number in Vial (cells/1ml) | 2.4 x 10^6 |
| Viability at cell freezing (%) | 98 | Antibiotics Used | free |
| Passage Number | Unknown (20 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:9,10 D13S317:8,10 D7S820:11 D16S539:9,12 VWA:16,17 TH01:7,9 AM:X TPOX:8,12 CSF1PO:7,12 |
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| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 91 | Additional information |