JCRB0172 RMG-I

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0172 Cell Name   RMG-I
Profile   Human tumor cell line from ovarian mesonephroid adenocarcinoma. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   34
Identity   available Tissue for Primary Cancer   ovary
Case history   ovarian mesonephroid adenocarcinoma Metastasis   
Tissue Metastasized    Genetics   tumor markers = CA-125, TPA, BFP, Behta-micro-globulin. Mode of chrosomosome = 47.
Life Span   infinite Crisis PDL   
Morphology   not informed Character   Doubling time = 60 hrs. Colonyformation rate = 81 %.
Classify   tumor Established by   Nozawa,S. et.al.
Registered by   Nozawa,S. Regulation for Distribution   
Comment   Cell culture initiated by cell dispersion culture method from suspension cells in ascites and cloned later. Passage is over 104 when deposited to the FDSC. Cells were uniqure confirmed by the STR-PCR method. Year   1990
Medium   Ham's F12 medium 90% with 10% fetal bovine serum. Methods for Passages   Cells are subcultured after treatment with 0.02% EDTA and 2.5 % trypsin.
Cell Number on Passage   7 x 10^4 cells/sq.cm. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   ovary
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1662
Reference
Pubmed id:3154025Establishment of a human ovarian clear cell carcinoma cell line (RMG-I) and its single cell cloning--with special reference to the stem cell of the tumor.
Nozawa S,Tsukazaki K,Sakayori M,Jeng CH,Iizuka R
Hum Cell. 1988 Dec;1(4):426-35

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LOT Information

Cell No.   JCRB0172 Cell Name   
LOT No.   07052011 Lot Specification   distribution
Medium   Ham's F12 medim with 10% fetal bovine serum (FBS: GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 30 hrs. Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   72.1 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0172 Cell Name   RMG-1
LOT No.   06212001 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   2.2 x 10^5 cells/ml Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA at room temperature for 5 minutes.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   92.7 Antibiotics Used   free
Passage Number   P12* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0172 Cell Name   RMG-I
LOT No.   07142017 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.7 - 1.4 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   3.4 x 10^6
Viability at cell freezing (%)   88.0 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
Viability immediately after thawing (%)   88.0 Additional information   
Cell No.   JCRB0172 Cell Name   RMG-I
LOT No.   02042020 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 1.1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 33 hrs. Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:8,12
D7S820:11
D16S539:9,10
VWA:17,18
TH01:6,7
AM:X
TPOX:11
CSF1PO:10
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
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