JCRB0174.0 NCC16-P11

Cell information

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Supplements for the Serum free culture medium

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0174.0 Cell Name   NCC16-P11
Profile   Normal human endo-cervical columner cells immortalized by HPV16 virus genome and no tumor formation observed in nude mice. Growth is not dependent to EGF. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   uterine cervix
Case history   normal cervical-endo, columner cells, immortalized by HPV16. Growth is not dependent to EGF. Metastasis   
Tissue Metastasized    Genetics   E6/E7 positive. Telomerase positive.
Life Span   infinite Crisis PDL   
Morphology   Cells are round but attach to dishes. Character   Columnar epitherium cells immortalized. No tumor formation observed in nude mice.
Classify   immortalized Established by   Yasumoto,S.
Registered by   Yasumoto,S. Regulation for Distribution   Not defined.
Comment   The NCC16-P11 was confirmed to be unique on 07/30/2001 while the NCC16 originally deposited was crosscontaminated with the PHK16-0b. The original NCC16 was rejected to resistrate to the JCRB cell bank. Year   1999
Medium   MCDB153 medium (0.03 mM Ca2+) with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 40-70 ug/ml BPE. MCDB153 with < 0.5% FCS was also used in original report. Methods for Passages   Cells are harvested at 10x10^4 cells/sq.cm. after treatment with 0.1% trypsin and 0.01% EDTA.
Cell Number on Passage   4.5 x 10^4 cells/sq.cm. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   endocervix
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3044
Reference
Pubmed id:9310137 Two distinct human uterine cervical epithelial cell lines established after transfection with human papillomavirus 16 DNA.
Ohta Y,Tsutsumi K,Kikuchi K,Yasumoto S
Jpn J Cancer Res. 1997 Jul;88(7):644-51
Pubmed id:8710384 Telomerase activity in normal human epithelial cells.
Yasumoto S,Kunimura C,Kikuchi K,Tahara H,Ohji H,Yamamoto H,Ide T,Utakoji T
Oncogene. 1996 Jul 18;13(2):433-9

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LOT Information

Cell No.   JCRB0174.0 Cell Name   NCC16-P11
LOT No.   01052011 Lot Specification   distribution
Medium   MCDB153 medium with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 50 ug/ml bovine pituitary extract. (serum-free) Temperature   37 C
Cell Density at Seeding   0.92-4.3x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.01% EDTA at 10x10^4 cells/sq.cm.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.01x10^6
Viability at cell freezing (%)   99.2 Antibiotics Used   free
Passage Number   p26 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:12
D7S820:10,11
D16S539:9,10
VWA:16,19
TH01:6,9.3
AM:X
TPOX:8
CSF1PO:9,10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0174.0 Cell Name   NCC16-P11
LOT No.   08032001 Lot Specification   distribution
Medium   MCDB153 medium with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 50 ug/ml bovine pituitary extract. (serum-free) Temperature   37 C
Cell Density at Seeding   1.0 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.1% trypsin and 0.01% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   80.1 Antibiotics Used   free
Passage Number   P20 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human, not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Equal volume of the culture medium with FM-1 medium containing 10% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0174.0 Cell Name   NCC16-P11
LOT No.   07182001 Lot Specification   distribution
Medium   MCDB153 medium with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 50 ug/ml bovine pituitary extract. (serum-free) Temperature   37 C
Cell Density at Seeding   1.0 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.1% trypsin and 0.01 % EDTA. Subculture every 7-10 days. T75 flasks used.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   81.0 Antibiotics Used   free
Passage Number   P19 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cells in the serum free culture was mixed with equivolume of the FM-1 freezing medium which is containing final 10% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0174.0 Cell Name   NCC16-P11
LOT No.   04102018 Lot Specification   distribution
Medium   MCDB153 medium(0.03 mM Ca2+) with 5ug/ml insulin, 0.5 ug/ml hydrocortisone,10 ug/ml transferin, 0.1 nM phosphorylethanolamine,0.1mM ethanolamine,10 ng/ml EGF and 40-70 ug/ml Temperature   37 C
Cell Density at Seeding   0.3-3.2 x 10^4 cells/sq.cm. Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.01% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   90.00 Antibiotics Used   
Passage Number   p26 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:12
D7S820:10,11
D16S539:9,10
VWA:16,19
TH01:6,9.3
AM:X
TPOX:8
CSF1PO:9,10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   86.2
Additional information   
Images
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