JCRB0232.1 SKG-IIIb

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0232.1 Cell Name   SKG-IIIb
Profile   Human tumor cell line with non-keratinizing type squamous cell carcinoma from cervical tumor. Temperature sensitive growth was observed. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   38
Identity   not done Tissue for Primary Cancer   uterine cervix
Case history   large cell non-keratinizing type squamous cell carcinoma Metastasis   
Tissue Metastasized    Genetics   tumor markers, TA-4, hCG, behta-hCG, PP7
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   The cell line was expected to be derived from an intermedially differentiated squamous cell carcinoma. Growth of the cell line SKG-IIIb was suppressed at 39 C.
Classify   tumor Established by   Udagawa,Y. & Nozawa,S.
Registered by   Nozawa,S. Regulation for Distribution   
Comment   The cell line was contaminated by mycoplasmas when deposited to the cell bank and they were eliminated in FDSC(JCRB). Year   1990
Medium   Ham's F12 (pH.7.2) medium with 10% fetal calf serum. Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Cell Number on Passage   1.5 x 10^5 cells/sq.cm. Race   Japanese
CO2 Conc.    Tissue Sampling   cervix of uterus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2795
Reference
Pubmed id:11092985 Regulation of dihydropyrimidine dehydrogenase and pyrimidine nucleoside phosphorylase activities by growth factors and subsequent effects on 5-fluorouracil sensitivity in tumor cells.
Ueda M,Kitaura K,Kusada O,Mochizuki Y,Yamada N,Terai Y,Kumagai K,Ueki K,Ueki M
Jpn J Cancer Res. 2000 Nov;91(11):1185-91
Pubmed id:10545752 Biological implications of growth factors on the mechanism of invasion in gynecological tumor cells.
Ueda M,Ueki M,Terai Y,Ueki K,Kumagai K,Fujii H,Yoshizawa K,Nakajima M
Gynecol Obstet Invest. 1999;48(3):221-8
Pubmed id:9355022 Inhibitory effects of ubenimex (bestatin) on the invasion of uterine cervical carcinoma cells and their production and activation of gelatinase A.
Ueda M,Ueki M,Fujii H,Yoshizawa K,Nakajima M
J Med. 1997;28(3-4):175-90
Pubmed id:9378537 Stimulatory effects of EGF and TGF-alpha on invasive activity and 5'-deoxy-5-fluorouridine sensitivity in uterine cervical-carcinoma SKG-IIIb cells.
Ueda M,Ueki M,Terai Y,Morimoto A,Fujii H,Yoshizawa K,Yanagihara T
Int J Cancer. 1997 Sep 17;72(6):1027-33
Pubmed id:2212803 [The mechanism of cytotoxicity of monoclonal antibodies to gynecological cancer cell-lines].
Iida S,Nozawa S
Nihon Sanka Fujinka Gakkai Zasshi. 1990 Jul;42(7):667-74
Pubmed id:3122483 Anticellular activities of human fibroblast interferon beta and human recombinant interferon gamma. Cytologic changes in a cervical cancer cell line.
Iwasaka T,Yoshimura T,Ohkuma Y,Tsugitomi H,Sugimori H
Acta Cytol. 1987 Nov-Dec;31(6):941-5
Pubmed id:3032185 Conservation of E6 and E7 regions of human papillomavirus types 16 and 18 present in cervical cancers.
Takebe N,Tsunokawa Y,Nozawa S,Terada M,Sugimura T
Biochem Biophys Res Commun. 1987 Mar 30;143(3):837-44
Pubmed id:3794454 [Sensitivity to etoposide in cultured cells from cervical squamous cell carcinoma].
Maeda T,Ueda M,Yamada T,Kubo H,Okamura S,Sano T,Ueki M,Sugimoto O
Nihon Sanka Fujinka Gakkai Zasshi. 1986 Nov;38(11):2050-6
Pubmed id:6209350 [Peplomycin sensitivity of various types of human gynecological cultured tumor cells].
Ueda M,Nozawa S,Ueki M,Sano T,Nagai S,Sugimoto O,Kurihara S
Nihon Sanka Fujinka Gakkai Zasshi. 1984 Oct;36(10):1867-76
Pubmed id:6699456 [Biological properties of two newly established cell lines (SKG-3a,3b) from a human uterine cervical epidermoid carcinoma].
Udagawa Y,Nozawa S,Tsukazaki K,Ohta H,Akiba T,Kurihara S
Nihon Sanka Fujinka Gakkai Zasshi. 1984 Feb;36(2):237-46
Pubmed id:6600963 Newly established uterine cervical cancer cell line (SKG-III) with Regan isoenzyme, human chorionic gonadotropin beta-subunit, and pregnancy-specific beta 1-glycoprotein phenotypes.
Nozawa S,Udagawa Y,Ohta H,Kurihara S,Fishman WH
Cancer Res. 1983 Apr;43(4):1748-60
Pubmed id:3029293

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LOT Information

Cell No.   JCRB0232.1 Cell Name   SKG-IIIb
LOT No.   08162001 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   6.0 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA for 5 minutes at 37 C. Subculture once a week and medium change twice a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   96.3 Antibiotics Used   free
Passage Number   P15* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:12
D7S820:10,12
D16S539:9,11
VWA:16
TH01:6,9
AM:X
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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