JCRB0176 RKN

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0176 Cell Name   RKN
Profile   Human tumor cell line from myoma ovarii. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   45
Identity   available Tissue for Primary Cancer   ovary
Case history   myoma ovarii Metastasis   
Tissue Metastasized    Genetics   mode of chromosome=74
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Doubling time=31.2 hours.
Classify   tumor Established by   Tsukazaki,K. & Nozawa,S.
Registered by   Nozawa,S. Regulation for Distribution   
Comment   Cells were contaminated by mycoplasmas when deposited. They were removed by FDSC before sending to the JCRB. Year   1990
Medium   Han's F12 medium 90% with 10% fetal bovine serum. Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   1.2 x 10^5 cells/sq.cm. Race   Japanese
CO2 Conc.    Tissue Sampling   ovary
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3156
Reference
Pubmed id:6512337 [Correlation between cell cycle and cell morphology of RKN cells].
Nozawa S,Widjaja T,Arai H,Tsukazaki K,Nagai S,Kurihara S,Sugishita T,Tenjin Y
Nihon Sanka Fujinka Gakkai Zasshi. 1984 Aug;36(8):1213-22

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LOT Information

Cell No.   JCRB0176 Cell Name   RKN
LOT No.   09142010 Lot Specification   distribution
Medium   Ham's F12 medium with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.24x10^6
Viability at cell freezing (%)   97.4 Antibiotics Used   free
Passage Number   p19* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:8,9
D7S820:8,10
D16S539:11
VWA:17
TH01:6
AM:X
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0176 Cell Name   RKN
LOT No.   08272001 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (RB52405). Temperature   37 C
Cell Density at Seeding   5.0 x 10^3 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDAT for 1-2 minutes at room temperature.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   96.2 Antibiotics Used   free
Passage Number   P19* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
RFLP   Cells were unique. Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0176 Cell Name   RKN
LOT No.   07082014 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.3 - 7.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   Unknown (19 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   96.1
Additional information   
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