JCRB0172.1 RMG-II

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0172.1 Cell Name   RMG-II
Profile   Human ovarian mesonephroid adenocarcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   53
Identity   available Tissue for Primary Cancer   ovary
Case history   ovarian mesonephroid adenocarcinoma Metastasis   
Tissue Metastasized    Genetics   Chromosomes are observed as triploid. Tumor markers, CA-125, TPA were observed.
Life Span   infinite Crisis PDL   
Morphology    Character   Doubling time is around 58 hours. Passage number is over 73 at deposit.
Classify   tumor Established by   Yajima,M. et.al.
Registered by   Nozawa,S. Regulation for Distribution   
Comment   Cells confirmed to be registrated and ampules for distribution are under preparation. Year   1990
Medium   Ham's F-12 medium with 10 % fetal calf serum. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin.
Cell Number on Passage   8.0 x 10^4 cells/sq.cm. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   ovary
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2803
Reference
Pubmed id:1715339A new CA125-like antigen (CA602) recognized by two monoclonal antibodies against a newly established ovarian clear cell carcinoma cell line (RMG-II).
Nozawa S,Yajima M,Sasaki H,Tsukazaki K,Aoki D,Sakayori M,Udagawa Y,Kobayashi T,Sato I,Furusako S
Jpn J Cancer Res. 1991 Jul;82(7):854-61
Pubmed id:2730079[Monoclonal antibodies raised against cultured cells and their application in cancer diagnosis].
Nozawa S,Udagawa Y
Gan To Kagaku Ryoho. 1989 Apr;16(4 Pt 2-3):1858-63

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LOT Information

Cell No.   JCRB0172.1 Cell Name   RMG-II
LOT No.   07132004 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   0.7 - 1.3 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   84 hrs Cell Number in Vial (cells/1ml)   7.6 x 10^5
Viability at cell freezing (%)   97.6 Antibiotics Used   free
Passage Number   P23* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0172.1 Cell Name   RMG-II
LOT No.   07012013 Lot Specification   distribution
Medium   Ham's F-12 medium with 10 % fetal calf serum (FBS; GIBCO Cat. # 10099). Temperature   37C
Cell Density at Seeding   2.2-4.9 x 10^4 cells/mL Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.3 x 10^6 (cells/mL)
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P22* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   51.6 Additional information   
Cell No.   JCRB0172.1 Cell Name   RMG-II
LOT No.   02032016 Lot Specification   distribution
Medium   Ham's F-12 medium with 10 % fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   2.2 - 10 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 51 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   73 Antibiotics Used   free
Passage Number   Unknown (23 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DSMO, 20% FBS -Ham's F-12 CO2 Conc.   5%
Viability immediately after thawing (%)   73 Additional information   
Cell No.   JCRB0172.1 Cell Name   RMG-II
LOT No.   02252020 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   3 - 8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 3 days Cell Number in Vial (cells/1ml)   7.6 x 10^5
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
Viability immediately after thawing (%)   66 Additional information   
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