JCRB0179 SNG-M

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0179 Cell Name   SNG-M
Profile   Intermedicately differentiated human adenocarcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   52
Identity   not done Tissue for Primary Cancer   uterus
Case history   uterus tumor Metastasis   No
Tissue Metastasized    Genetics   Mordal No. of chromosome is 40, Colony formation rate is 40%, Doubling time is 30 hours indicated by Nozawa,S.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   intermediately differentiated adenocarcinoma
Classify   tumor Established by   Ishiwata,I. and Nozawa,S.
Registered by   Nozawa,S. Regulation for Distribution   
Comment   The cells are responded to the progesterone. Mycoplasmas were decontaminated by MC210 in FDSC, 1990. Year   1990
Medium   Ham's F12 medium 80% with 20% fetal bovine serum. Methods for Passages   Cells harvested after treatment with 0.25% trypsin.
Cell Number on Passage   2 x 10^5 cells/sq.cm. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   metastatic lymph node
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1707
Reference
Pubmed id:10545752Biological implications of growth factors on the mechanism of invasion in gynecological tumor cells.
Ueda M,Ueki M,Terai Y,Ueki K,Kumagai K,Fujii H,Yoshizawa K,Nakajima M
Gynecol Obstet Invest. 1999;48(3):221-8
Pubmed id:8641991Effects of sex steroids and growth factors on migration and invasion of endometrial adenocarcinoma SNG-M cells in vitro.
Ueda M,Fujii H,Yoshizawa K,Abe F,Ueki M
Jpn J Cancer Res. 1996 May;87(5):524-33
Pubmed id:7758945Trophinin and tastin, a novel cell adhesion molecule complex with potential involvement in embryo implantation.
Fukuda MN,Sato T,Nakayama J,Klier G,Mikami M,Aoki D,Nozawa S
Genes Dev. 1995 May 15;9(10):1199-210
Pubmed id:2787771Adoptive cellular immunotherapy to the endometrial carcinoma cell line xenografts in nude mice.
Shimizu H,Inoue M,Tanizawa O
Gynecol Oncol. 1989 Aug;34(2):195-9
Pubmed id:2746066[Glycosphingolipids in human uterine endometrium: expression of sulfoglycolipids and its fluctuation during menstrual cycle].
Kubushiro K
Nihon Sanka Fujinka Gakkai Zasshi. 1989 Apr;41(4):397-404
Pubmed id:3027213[Reorganization of human ovarian adenocarcinoma cells and endometrial adenocarcinoma cells cultured in double-layered floating collagen gels].
Tsumura N,Kudo R,Sawada N
Nihon Sanka Fujinka Gakkai Zasshi. 1986 Dec;38(12):2157-70
Pubmed id:3840512[Studies on the significance of PLD repair for radioresistance in human uterine adenocarcinoma cells].
Mitsunaga S
Nihon Sanka Fujinka Gakkai Zasshi. 1985 Sep;37(9):1859-65
Pubmed id:2580923[Experimental studies on combination chemotherapy based on cell cycle analysis].
Tsuboi T
Nihon Sanka Fujinka Gakkai Zasshi. 1985 Apr;37(4):629-36
Pubmed id:7276650[Studies on temperature sensitivity of human endometrial adenocarcinoma cells in vitro (author's transl)].
Udagawa Y
Nihon Sanka Fujinka Gakkai Zasshi. 1981 Aug;33(8):1235-44
Pubmed id:642036Effects of progesterone on human endometrial carcinoma cells in vivo and in vitro.
Ishiwata I,Udagawa Y,Okumura H,Nozawa S
J Natl Cancer Inst. 1978 May;60(5):947-54
Pubmed id:858140Development and characterization of established cell lines from primary and metastatic regions of human endometrial adenocarcinoma.
Ishiwata I,Nozawa S,Inoue T,Okumura H
Cancer Res. 1977 Jun;37(6):1777-85

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LOT Information

Cell No.   JCRB0179 Cell Name   SNG-M
LOT No.   07212009 Lot Specification   distribution
Medium   Ham's F12 medium with 20% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   7 - 20 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx 52 hrs Cell Number in Vial (cells/1ml)   4.2 x 10^6
Viability at cell freezing (%)   67.7 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - F12 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0179 Cell Name   SNG-M
LOT No.   11152001 Lot Specification   distribution
Medium   Ham's F12 medium with 20% fetal bovine serum(Intergen RB52405). Temperature   37 C
Cell Density at Seeding   5.5 x 10^3 cells/sq.cm. Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Medium change once a week and subculture once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   94.6 Antibiotics Used   free
Passage Number   P16* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0179 Cell Name   SNG-M
LOT No.   10062017 Lot Specification   distribution
Medium   Ham's F12 medium with 20% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   2.3 - 5.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   78.6 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
Viability immediately after thawing (%)   78.6 Additional information   
Cell No.   JCRB0179 Cell Name   SNG-M
LOT No.   11082022 Lot Specification   distribution
Medium   Ham's F-12 medium with 20% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   2.3 - 6.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 23 hrs. Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   Unknown (17 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,13
D13S317:8,10,11
D7S820:10,11,12
D16S539:10,12
VWA:16,17,18,20
TH01:6,7
AM:X
TPOX:8,9
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F-12 CO2 Conc.   5%
Viability immediately after thawing (%)   85 Additional information   
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