JCRB0180 GAK

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0180 Cell Name   GAK
Profile   Human malignant melanoma cell line with the production of 5-S-cysteinyldopa. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   86
Identity   available Tissue for Primary Cancer   melanoma
Case history   malignant melanoma Metastasis   Yes
Tissue Metastasized   inguinal lymph node Genetics   Mode of chromosome is 67. DT=72 hours. Tumor marker = 5-S-cysteinyldopa.
Life Span   infinite Crisis PDL   
Morphology    Character   The cell line GAK has an activity of 5-S-CD production.
Classify   tumor Established by   Nozawa,S. and Ohta,H.
Registered by   Nozawa,S. Regulation for Distribution   
Comment    Year   1990
Medium   Ham's F12 medium with 20% fetal bovine serum. Methods for Passages   Cells harvested after treatment with 0.25% trypsin.
Cell Number on Passage   8.4 x 10^4 cells / sq.cm. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   inguinal lymph node
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1225
Reference
Pubmed id:6431036[A newly established melanoma cell line (GAK) with 5-S-cysteinyldopa phenotype].
Nozawa S,Ohta H,Tsukazaki K,Udagawa Y,Kurihara S,Kano S
Nihon Sanka Fujinka Gakkai Zasshi. 1984 Jun;36(6):883-92

Images



LOT Information

Cell No.   JCRB0180 Cell Name   GAK
LOT No.   02242012 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (GIBCO Cat. # 10099). Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   9.6 x 10^5
Viability at cell freezing (%)   88.3 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0180 Cell Name   GAK
LOT No.   11052001 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   2.0 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.02% EDTA and 0.25% trypsin (GIBCO BRL). Medium change once a week and subculture every five days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   98.2 Antibiotics Used   free
Passage Number   P20 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5 % DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0180 Cell Name   GAK
LOT No.   02082022 Lot Specification   distribution
Medium   Ham's F-12 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   0.9 - 1.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 38 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F-12 CO2 Conc.   5%
Viability immediately after thawing (%)   92 Additional information   
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