JCRB0183 QGP-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0183 Cell Name   QGP-1
Profile   Carcinoembryonic antigen (CEA)-producing human pancreatic ilet cell carcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   61-year-old
Identity   available Tissue for Primary Cancer   pancreas
Case history   pancreatic carcinoma of islet cell (somatostatinoma) Metastasis   No
Tissue Metastasized    Genetics   Modal chromosome number is 50. CEA production.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cell line produces carcinoembryonic antigen (CEA). Cells create irregular cell aggregates, and grow as a confluent monolayer with piling up of cells in some area. Population doubling time is 3.5 days. Useful to study the regulation mechanisms of SRIF.
Classify   tumor Established by   Kaku,M. et.al.
Registered by   Iguchi,H. Regulation for Distribution   
Comment   Cells are observed to be unique from the STR-PCR analysis. Year   2001
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Trypsin treatment.
Cell Number on Passage   5 x 10^5 cells/35mm dish Race   Japanese
CO2 Conc.    Tissue Sampling   islet cell of pancreas
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3143
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1298534
Reference
Pubmed id:1971195A somatostatin-secreting cell line established from a human pancreatic islet cell carcinoma (somatostatinoma): release experiment and immunohistochemical study.
Iguchi H,Hayashi I,Kono A
Cancer Res. 1990 Jun 15;50(12):3691-3
Pubmed id:7227711Establishment of a carcinoembryonic antigen-producing cell line from human pancreatic carcinoma.
Kaku M,Nishiyama T,Yagawa K,Abe M
Gan. 1980 Oct;71(5):596-601

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LOT Information

Cell No.   JCRB0183 Cell Name   
LOT No.   04112012 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   3 - 5 x 10^5 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage before full confluency. Recommended passage interval is 4 days.
Doubling Time   approx. 42 hrs. Cell Number in Vial (cells/1ml)   9.6 x 10^5
Viability at cell freezing (%)   85.7 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0183 Cell Name   
LOT No.   02162009 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. The growth is very slow 1-2 weeks after thawing.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   7.4 x 10^6
Viability at cell freezing (%)   93.5 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0183 Cell Name   
LOT No.   04152003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovie serum Temperature   
Cell Density at Seeding   2.0 x 10^5cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   DT=40-50hr Cell Number in Vial (cells/1ml)   5.0 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   
Passage Number   >P7 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS in RPMI1640 CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0183 Cell Name   QGP-1
LOT No.   05312016 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 2.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 74 hrs. Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   94 Additional information   
Cell No.   JCRB0183 Cell Name   QGP-1
LOT No.   02262018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.4 - 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 43 hrs. Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   89 Additional information   
Cell No.   JCRB0183 Cell Name   QGP-1
LOT No.   03182021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.3 - 3.0 x 10^5 cells/mL Methods for Passages   Cells were harvested aftrer treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 44 hrs. Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   97.3 Additional information   
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