JCRB0188 KYSE30
Cell information
Important Notice(s)On the MTA of KYSE series cell lines with Kyoto University (for profit organizations)
Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0188 | Cell Name | KYSE30 |
|---|---|---|---|
| Profile | Well differentiated human suamous cell carcinoma from esophageal cancer. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 64 |
| Identity | available | Tissue for Primary Cancer | esophagus |
| Case history | esophageal cancer, middle intrathoracic esophagus, well differentiated squamous cell carcinoma. | Metastasis | No |
| Tissue Metastasized | Genetics | Mode of chromosome number = 90-100. DNA fingerprint indicated the cell line was unique. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Cell line was established from inplanted tumor of nude mice (only initial passage). |
| Classify | tumor | Established by | Shimada,Y. et.al. |
| Registered by | Shimada,Y. | Regulation for Distribution | Cite reference (Cancer, 69:277(1992)). Company users require MTA from originator. Only use in Japan |
| Comment | Distribution restricted to Japan(domestic use only). | Year | 2001 |
| Medium | Ham's F12/RPMI1640 medium containing 20% fetal calf serum for primary culture (original description). Current medium for culture: DMEM with 2% FBS. | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5 % | Tissue Sampling | esophagus |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:9033652 | Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines. Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K Int J Cancer. 1997 Feb 7;70(4):437-42 |
| Pubmed id:8575860 | Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors. Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K Int J Cancer. 1996 Jan 26;65(3):372-6 |
| Pubmed id:1728357 | Characterization of 21 newly established esophageal cancer cell lines. Shimada Y,Imamura M,Wagata T,Yamaguchi N,Tobe T Cancer. 1992 Jan 15;69(2):277-84 |
| Images |
|---|
    |
| Movies |
|---|
|
LOT Information
| Cell No. | JCRB0188 | Cell Name | |
|---|---|---|---|
| LOT No. | 04072004 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 2% fetal bovine serum (FBS lot; GIBCO 3762782S) | Temperature | 37 C |
| Cell Density at Seeding | 5-6 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | ca. 2.2 days | Cell Number in Vial (cells/1ml) | 2.3 x 10^6 |
| Viability at cell freezing (%) | 95.3 | Antibiotics Used | free |
| Passage Number | 5 at bank | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), AST and MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% DBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0188 | Cell Name | KYSE30 |
|---|---|---|---|
| LOT No. | 03082013 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 2% fetal bovine serum (FBS, GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 2.3 - 6.7 x 10^4 cells / mL | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1.4 x 10^6 cells / m | |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | p5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | yes | Exoteric Gene | NT |
| Medium for Freezing | 10% DMSO, 20% FBS, DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0188 | Cell Name | KYSE30 |
|---|---|---|---|
| LOT No. | 04042019 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 2% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 0.3 - 1.4 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 20 hrs. | Cell Number in Vial (cells/1ml) | 2.7 x 10^6 |
| Viability at cell freezing (%) | 98.8 | Antibiotics Used | free |
| Passage Number | Unknown (5 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 98.5 | Additional information |