JCRB0188 KYSE30

Cell information

Important Notice(s)

KYSEシリーズ細胞株の民間企業への分譲について

On the MTA of KYSE series cell lines with Kyoto University (for profit organizations)

KYSEシリーズ細胞株の使用について

Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0188 Cell Name   KYSE30
Profile   Well differentiated human suamous cell carcinoma from esophageal cancer. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   64
Identity   available Tissue for Primary Cancer   esophagus
Case history   esophageal cancer, middle intrathoracic esophagus, well differentiated squamous cell carcinoma. Metastasis   No
Tissue Metastasized    Genetics   Mode of chromosome number = 90-100. DNA fingerprint indicated the cell line was unique.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cell line was established from inplanted tumor of nude mice (only initial passage).
Classify   tumor Established by   Shimada,Y. et.al.
Registered by   Shimada,Y. Regulation for Distribution   Cite reference (Cancer, 69:277(1992)). Company users require MTA from originator. Only use in Japan
Comment   Distribution restricted to Japan(domestic use only). Year   2001
Medium   Ham's F12/RPMI1640 medium containing 20% fetal calf serum for primary culture (original description). Current medium for culture: DMEM with 2% FBS. Methods for Passages   0.25% trypsin and 0.02% EDTA
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   esophagus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1351
Reference
Pubmed id:9033652 Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines.
Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K
Int J Cancer. 1997 Feb 7;70(4):437-42
Pubmed id:8575860 Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors.
Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K
Int J Cancer. 1996 Jan 26;65(3):372-6
Pubmed id:1728357 Characterization of 21 newly established esophageal cancer cell lines.
Shimada Y,Imamura M,Wagata T,Yamaguchi N,Tobe T
Cancer. 1992 Jan 15;69(2):277-84

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LOT Information

Cell No.   JCRB0188 Cell Name   
LOT No.   04072004 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 2% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   5-6 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 2.2 days Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   5 at bank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST and MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% DBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0188 Cell Name   KYSE30
LOT No.   03082013 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 2% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2.3 - 6.7 x 10^4 cells / mL Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.4 x 10^6 cells / m
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   yes Exoteric Gene   NT
Medium for Freezing   10% DMSO, 20% FBS, DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0188 Cell Name   KYSE30
LOT No.   04042019 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 2% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.3 - 1.4 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 20 hrs. Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   98.8 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   98.5
Additional information   
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