JCRB0189 KYSE50
Cell information
Important Notice(s)On the MTA of KYSE series cell lines with Kyoto University (for profit organizations)
Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan
Cell type:general cells (View Pricing Information)
JCRB No. | JCRB0189 | Cell Name | KYSE50 |
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Profile | Stop providing for the issue of quality control. (Please contact us for more information) Poorly differentiated human squamous cell carcinoma from esophageal cancer. | Other Name | |
Animal | human | Strain | |
Genus | Homo | Species | sapiens |
Sex | M | Age | 58 |
Identity | available | Tissue for Primary Cancer | esophagus |
Case history | esophageal cancer, lower intrathoracic esophagus, poorly differentiated squamous cell carcinoma. | Metastasis | No |
Tissue Metastasized | Genetics | Mode of chromosome number = 105-120. DNA fingerprint indicated the cell line was unique. | |
Life Span | infinite | Crisis PDL | |
Morphology | epithelial-like | Character | Cell line was established from inplanted tumor of nude mice (only initial passage). |
Classify | Established by | Shimada,Y. et.al. | |
Registered by | Shimada,Y. | Regulation for Distribution | Cite reference (Cancer, 69:277(1992)). Company users require MTA from the originator. See additional |
Comment | Distribution restricted to Japan(domestic use only). | Year | 2001 |
Medium | Dulbecco's modified Eagle's medium with 2% fetal calf serum. | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
Cell Number on Passage | Race | Japanese | |
CO2 Conc. | 5 % | Tissue Sampling | esophagus |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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BKV | - | HIV-1 | - | ||||||
JCV | - | HIV-2 | - | ||||||
ADV | - | HPV18 | - | ||||||
Notes |
Reference | |
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Pubmed id:9033652 | Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines. Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K Int J Cancer. 1997 Feb 7;70(4):437-42 |
Pubmed id:8575860 | Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors. Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K Int J Cancer. 1996 Jan 26;65(3):372-6 |
Pubmed id:1728357 | Characterization of 21 newly established esophageal cancer cell lines. Shimada Y,Imamura M,Wagata T,Yamaguchi N,Tobe T Cancer. 1992 Jan 15;69(2):277-84 |
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