-Detailed Information [JCRB0189]-

JCRB0189 KYSE50

Cell information

Important Notice(s)

当該細胞株は、利用者が私企業に所属する場合、京都大学との間で個別にマテリアルトランスファーアグリーメント (MTA) を取り交わすよう依頼を受けております。従って、企業ユーザーの方は分譲の依頼に先立ち、下記、細胞樹立者の嶋田先生に連絡して京都大学との間でＭＴＡを取り交わし、そのコピーを貼付して分譲依頼を行ってください。その際、実験目的等の情報についてお尋ねする場合があります。

なお、嶋田先生に連絡をする際、ＪＣＲＢ番号、細胞名を明確にお伝えください。

連絡先：

〒606-8501
京都市左京区吉田下阿達町46-29
京都大学薬学研究科
ナノバイオ医薬創成科学
嶋田 裕
Tel: 075-753-9558
Fax: 075-753-9557
yshimada@pharm.kyoto-u.ac.jp

これは京都大学の基準が適応されてされております。
アカデミックユーザーの場合はこの取扱の必要はありません。直接分譲依頼をしてください。


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On the MTA of KYSE series cell lines with Kyoto University (for profit organizations)

KYSE series of esophageal cancer-derived cell lines were developed by 
Dr. Shimada at Kyoto University. Profit-organizations (company users) 
are required to contract MTA (material transfer agreement) with Kyoto
University prior to the ordering of KYSE series cell lines.

Please contact to Dr. Shimada and conclude MTA with Kyoto University. 
In ordering of KYSE cell lines, please send us the copy of MTA with 
Request and Agreement Form (Form A).

Contact information of Dr. Shimada:

Dr. Yutaka Shimada
Department of Nanobio Drug Discovery
Graduate School of Pharmaceutical Sciences
Kyoto University
Yoshida Shimoadachicho 46-29
Kyoto 606-8501, Japan
Tel: 075-753-9558
Fax: 075-753-9557
yshimada@pharm.kyoto-u.ac.jp

In contact to Dr. Shimada, please clarify the JCRB number and name 
of cell line.

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KYSEシリーズ細胞株の使用について


−　KYSEシリーズ 食道がん由来細胞株の使用について　−

１．KYSEシリーズ細胞株の分譲は日本国内に限定することとなりました。

２．KYSEシリーズ細胞株の分譲に際しては、別途お送りする「細胞株の使用に関する同意書」にて次の事項に同意していただきます。
　−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
　(1)．当該細胞株を用いた発表論文には、次に指定する細胞株の樹立に関する原著論文
　　　 （当該細胞に関するもの）を引用する（ただし、引用論文数に制約等があり、
　　　 引用することが困難な場合はこの限りではない）。



　　　　Shimada Y, Imamura M, Wagata T, Yamaguchi N and Tobe T.
　　　　Characterization of twenty one newly established esophageal cancer
　　　　cell lines.　Cancer, 69: 277-284,(1992）

　　　　Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K.
　　　　Characterization of p53 gene mutations in esophageal squamous cell 
　　　　carcinoma cell lines: increased frequency and different spectrum 
　　　　of mutations from primary tumors.
　　　　Int J Cancer. 65:372-376 (1996)

　　　　Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K.
　　　　Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) 
　　　　genes in 30 esophageal squamous-cell-carcinoma cell lines.
　　　　Int J Cancer. 70:437-442 (1997)


　(2)．当該細胞株を用いて研究成果が得られた場合（論文や発表学会など）には、別刷り
　　　（あるいは論文のPDF)、学会要旨のコピー等を細胞株樹立者へ送付する。

　(3)．当該細胞株の第三者への配布は固く禁止する。
　−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−

３．KYSEシリーズ細胞株の民間企業への分譲に際しては、「細胞株の使用に関する同意書」とは別に、京都大学とのMTAの手交が
　　必要です。

(2014年12月15日 更新)

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Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

- Unavailability of KYSE series cell lines to oversea users outside of Japan -

The distribution of KYSE series esophageal cancer cell lines is limited to users in Japan. The JCRB/HSRRB cell bank is sorry for the unavailability of these cell lines to oversea researchers.

(Last updated May 14, 2008.)


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Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0189	Cell Name	KYSE50
Profile	Stop providing for the issue of quality control. (Please contact us for more information) Poorly differentiated human squamous cell carcinoma from esophageal cancer.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	58
Identity	available	Tissue for Primary Cancer	esophagus
Case history	esophageal cancer, lower intrathoracic esophagus, poorly differentiated squamous cell carcinoma.	Metastasis	No
Tissue Metastasized		Genetics	Mode of chromosome number = 105-120. DNA fingerprint indicated the cell line was unique.
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	Cell line was established from inplanted tumor of nude mice (only initial passage).
Classify		Established by	Shimada,Y. et.al.
Registered by	Shimada,Y.	Regulation for Distribution	Cite reference (Cancer, 69:277(1992)). Company users require MTA from the originator. See additional
Comment	Distribution restricted to Japan(domestic use only).	Year	2001
Medium	Dulbecco's modified Eagle's medium with 2% fetal calf serum.	Methods for Passages	0.25% trypsin and 0.02% EDTA
Cell Number on Passage		Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	esophagus
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1360
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	1298222

Reference
Pubmed id:9033652	Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines. Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K Int J Cancer. 1997 Feb 7;70(4):437-42
Pubmed id:8575860	Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors. Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K Int J Cancer. 1996 Jan 26;65(3):372-6
Pubmed id:1728357	Characterization of 21 newly established esophageal cancer cell lines. Shimada Y,Imamura M,Wagata T,Yamaguchi N,Tobe T Cancer. 1992 Jan 15;69(2):277-84

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Cell No.	JCRB0189	Cell Name	KYSE50
LOT No.	02182005	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 2% fetal bovine serum (FBS lot; GIBCO 3762782S)	Temperature	37 C
Cell Density at Seeding	1.5 - 2.0 x 10^5 cells/ml	Methods for Passages	200 units/ml trypsin and 0.01% EDTA
Doubling Time	ca. 2-3 days	Cell Number in Vial (cells/1ml)	1.4 x 10^6
Viability at cell freezing (%)	87.4	Antibiotics Used	free
Passage Number	P6 at bank	PDL
Sterility: MYCOPLASMA	+	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0189	Cell Name	KYSE50
LOT No.	02242015	Lot Specification	distribution
Medium	RPMI1640(GIBCO) and Ham's F12 medium(SiGMA)(1/1) with 2% heat inactivated fetal bovine serum(SIGMA12J396).	Temperature	37 C
Cell Density at Seeding	1.80-5.22x10^4cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 1mM EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.32x10^6
Viability at cell freezing (%)	85.7	Antibiotics Used
Passage Number	p10*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:11 D13S317:11 D7S820:10 D16S539:10,12 VWA:17,19 TH01:8 AM:X TPOX:11 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Cell Banker BLC-1(Nihon Zenyaku Industries)	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information	Acholeplasma has eliminated with Plasmocin(Final concentration 25 ug/ml). Expected viability (defrost ampoule) 88.0%.

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JCRB Cell Bank

JCRB0189 KYSE50

Cell information

LOT Information