JCRB0195 RP-1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0195 | Cell Name | RP-1 |
|---|---|---|---|
| Profile | Mouse cell line producing IgG1 against rat neutrophil. | Other Name | |
| Animal | mouse | Strain | BALB/c |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | antibody-producing hybridoma | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | Rat neutrophil specific IgG1 production. X-63 parent. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | This cell line is producing IgG1 against the rat neutrophil. No harmfull effect against the neutrophil. This IgG1 is used for the histochemical staining of the neutrophil. |
| Classify | hybridoma | Established by | Gotoh,S. and Sendo,F. et al. |
| Registered by | Sendo,F. et.al. | Regulation for Distribution | |
| Comment | Year | 2000 | |
| Medium | RPMI1640 medium with 10% fetal calf serum. or Iscov's modified Dulbecco's Medium with 10% fetal calf serum. | Methods for Passages | Dilution. Suspension cell culture. |
| Cell Number on Passage | Not defined. | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | ascites |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:9071321 | Long-term oral application of 5-bromo-2-deoxyuridine does not reliably label proliferating immune cells in the LEW rat. Jecker P,Beuleke A,Dressendörfer I,Pabst R,Westermann J J Histochem Cytochem. 1997 Mar;45(3):393-401 |
| Pubmed id:9375829 | Detailed analysis of phenotypes of macrophages infiltrating glomeruli in rat anti-Thy1 nephritis. Kobayashi H,Orikasa M,Naito M,Kawasaki K,Oite T,Kawachi H,Yamashita A,Takeya M,Nihei H,Shimizu F Nephron. 1997;77(3):333-9 |
| Pubmed id:2424975 | Enhancement of the expression of a rat neutrophil-specific cell surface antigen by activation with phorbol myristate acetate and concanavalin A. Gotoh S,Itoh M,Fujii Y,Arai S,Sendo F J Immunol. 1986 Jul 15;137(2):643-50 |
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LOT Information
| Cell No. | JCRB0195 | Cell Name | RP-1 |
|---|---|---|---|
| LOT No. | 12152001 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% heat inactivated (56 C, 30min.) fetal bovine serum(Intergen RB52405). | Temperature | 37 C |
| Cell Density at Seeding | 2-4 x 10^6 cells/ml | Methods for Passages | Simple dilution. Subculture every 3-4 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.3 x 10^6 |
| Viability at cell freezing (%) | 80.5 | Antibiotics Used | free |
| Passage Number | P2* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Culture medium containing 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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