JCRB0197 OSC-20
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0197 | Cell Name | OSC-20 |
|---|---|---|---|
| Profile | Human squamous cell carcinoma cell line from tongue metastasized to neck lymph node(left), which was cultured with serum free condition. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 59 |
| Identity | not done | Tissue for Primary Cancer | oral |
| Case history | cancer of tongue | Metastasis | Yes |
| Tissue Metastasized | cervical lymph node | Genetics | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Cells grew in cobblestone pattern. Electron microscopy revealed a presence of tonofilaments and desmosomes. This cells secreted GST-pai into the culture medium. |
| Classify | tumor | Established by | Yokoi,T. |
| Registered by | Yokoi,T. | Regulation for Distribution | |
| Comment | Serum free cell culture. When cells are treated with the trypsin inhibitor, 10^-7 M 6-amino-2-naphtyl p-guanidinbenzoate dimethanesulfonate(FUSAN) is used, cells should be washed by the serum free medium once. The FUSAN is toxic to the cell. | Year | 1990 |
| Medium | 1 to 1 mixture of Dulbecco's modified Eagle's medium and F-12 medium. Serum free cell culture. | Methods for Passages | Cells are washed three times with Ca-Mg free Hank's balanced salt solution(HBSS), then cells are treated with 0.01% trypsin and 0.01% EDTA in the HBSS. |
| Cell Number on Passage | 1 x 10^5 cells/60 mm dish | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | tongue |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Images |
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LOT Information
| Cell No. | JCRB0197 | Cell Name | |
|---|---|---|---|
| LOT No. | 11152011 | Lot Specification | distribution |
| Medium | 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium (serum-free) | Temperature | 37 C |
| Cell Density at Seeding | 5 - 7 x 10^4 cells/mL | Methods for Passages | 0.01% (or 0.02%) trypsin and 0.01% EDTA. Trypsin activity was terminated by 0.1% soybean trypsin inhibitor (Sigma type IS) |
| Doubling Time | approx. 49 hrs. | Cell Number in Vial (cells/1ml) | 1.1 x 10^6 |
| Viability at cell freezing (%) | 97.7 | Antibiotics Used | free |
| Passage Number | Unknown (10 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 0.1% methylcellulose in DMEM:F12 = 1:1 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0197 | Cell Name | |
|---|---|---|---|
| LOT No. | 02032003 | Lot Specification | distribution |
| Medium | 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium (serum-free) | Temperature | 37 C |
| Cell Density at Seeding | 5 x 10^4 cells/ml | Methods for Passages | 0.01% (or 0.02%) trypsin and 0.01% EDTA. Trypsin activity was terminated by 0.1% soybean trypsin inhibitor (Sigma type IS) |
| Doubling Time | 65 hrs | Cell Number in Vial (cells/1ml) | 1.3 x 10^6 |
| Viability at cell freezing (%) | 86.7 | Antibiotics Used | free |
| Passage Number | Unknown (10 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 0.1% methylcellulose in DMEM:F12 = 1:1 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0197 | Cell Name | OSC-20 |
|---|---|---|---|
| LOT No. | 07182019 | Lot Specification | distribution |
| Medium | 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium (serum-free). (Addition of 10% FBS is also possible.) | Temperature | 37 C |
| Cell Density at Seeding | 3.5 - 4.7 x 10^4 cells/mL | Methods for Passages | 0.02% trypsin and 0.01% EDTA in PBS (-). Trypsin activity was terminated by 0.1% soybean trypsin inhibitor (Sigma type IS) |
| Doubling Time | approx. 35 hrs. | Cell Number in Vial (cells/1ml) | 1.8 x 10^6 |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | Unknown (9 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:13,14 D13S317:8 D7S820:10 D16S539:9,13 VWA:18 TH01:9,9.3 AM:X TPOX:8,11 CSF1PO:12,13 |
|
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 0.1% methylcellulose in DMEM:F12 = 1:1 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 93.7 | Additional information |