JCRB0191 OCUG-1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0191 Cell Name   OCUG-1
Profile   Human gall bladder carcinoma cell line producing TA-4 antigen. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   43
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   gallbladder carcinoma Metastasis   Yes
Tissue Metastasized   peritoneal effusion Genetics   Chromosome number distribute in a broad range from 52 to 139.
Life Span   infinite Crisis PDL   
Morphology   clusters with monolayer cells Character   Only TA-4 antigen detected in the spent medium while high levele SLX, CA19-9, SPan-1 and TA-4 were observed in the serum of patient.
Classify   tumor Established by   Yamada,N. et.al.
Registered by   Hirakawa,K. Regulation for Distribution   
Comment   Cells are tumorigenic in nude mice. Poorly defferentiated adenocarcinoma. The cell line was identified to be a unique cell line by STR-PCR experiment (2002.June). Year   2002
Medium   Dulbecco's modified Eagle's medium 0.5mM pyruvate, 2mM glutamine and 10% fetal calf serum. Methods for Passages   
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   malignant peritoneal effusion
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3083
Reference
Pubmed id:21533512 Establishment and characterization of a new human gallbladder carcinoma cell line (OCUG-1) producing TA-4.
Yamada N,Chung Y,Ohtani H,Ikeda T,Onoda N,Sawada T,Nishiguchi Y,Hasuma T,Sowa M
Int J Oncol. 1997 Jun;10(6):1251-5

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LOT Information

Cell No.   JCRB0191 Cell Name   OCUG-1
LOT No.   11027111 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2.6 - 2.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   8.7 x 10^5
Viability at cell freezing (%)   89.7 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6Pd (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0191 Cell Name   
LOT No.   02052004 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 0.5 mM sodium pyruvate and 10% fetal bovine serum (FBS Lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   3 - 5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 2 days Cell Number in Vial (cells/1ml)   7.7 x 10^5
Viability at cell freezing (%)   93.3 Antibiotics Used   free
Passage Number   P8* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0191 Cell Name   OCUG-1
LOT No.   11042016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium 0.5 mM sodium pyruvate and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   2 - 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs. Cell Number in Vial (cells/1ml)   6.4 x 10^5
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   93
Additional information   
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