JCRB0192 OCUM-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0192 Cell Name   OCUM-1
Profile   Human gastric cancer cell line from poorly differentiated adenocarcinoma. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   38
Identity   available Tissue for Primary Cancer   stomach
Case history   gastric cancer, Borrmann type IV, poorly differentiated adenocarcinoma containing signet ring cells. Metastasis   Yes
Tissue Metastasized    Genetics   karyotype=mode 50, near diploid
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like with clustering Character   This cell line produces carcinoembryonic antigen(CEA), carbohydrate antigen 19-9 (CA19-9), cancer-associated antigen SPan-1, and expresses EGF receptor. Tumorigenic to nude mice (intramusclar transplantation).
Classify   tumor Established by   Kubo,T.
Registered by   Hirakawa,K. Regulation for Distribution   
Comment    Year   2002
Medium   Dulbecco's modified Eagle's medium with 0.5mM sodium pyruvate and 10% fetal bovine serum. Methods for Passages   Dilution (original description). Loosely attached cells were hravested by trypsinization (at cell bank).
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   stomach
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3084
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1298358
Reference
Pubmed id:1961183[Establishment and characterization of a new gastric cancer cell line (OCUM-1), derived from Borrmann type IV tumor].
Kubo T
Nihon Geka Gakkai Zasshi. 1991 Oct;92(10):1451-60
Pubmed id:1922102Molecular characterization of monoclonal anti-steroid antibodies: primary structures of the variable regions of seven antibodies specific for 17 alpha-hydroxyprogesterone or 11-deoxycortisol and their pH-reactivity profiles.
Sawada J,Mizusawa S,Terao T,Naito M,Kurosawa Y
Mol Immunol. 1991 Oct;28(10):1063-72

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LOT Information

Cell No.   JCRB0192 Cell Name   
LOT No.   11282012 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 0.5 mM sodium pyruvate and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.3 - 2.7 x 10^5 cells/mL Methods for Passages   Dilution (suspension culture)
Doubling Time   approx. 64 hrs Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0192 Cell Name   
LOT No.   09162009 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 0.5 mM sodium pyruvate and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2.1 - 3.4 x 10^5 cells/ml Methods for Passages   Original description is dilution. However, most of cells attached loosely to substratum, and these cells were harvested with 0.25% trypsin.
Doubling Time   49 hrs Cell Number in Vial (cells/1ml)   4.5 x 10^6
Viability at cell freezing (%)   96.9 Antibiotics Used   free
Passage Number   Unknown (12 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0192 Cell Name   
LOT No.   12222004 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 0.5 mM sodium pyruvate (final 1.5 mM) and 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1 - 5 x 10^5 cells/ml Methods for Passages   Original description is dilution. However, most of cells attached loosely to substratum, and these cells were harvested with 0.25% trypsin.
Doubling Time   approx. 38 hrs Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   99.1 Antibiotics Used   free
Passage Number   Unknown (P11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0192 Cell Name   OCUM-1
LOT No.   08172016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (low glucose) with 0.5 mM sodium pyruvate and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.7 - 2.4 x 10^5 cells/mL Methods for Passages   Dilution (suspension culture). In some cases, loosely attached cells appear, and they can be hravested by trypsinization.
Doubling Time   approx. 26 hrs. Cell Number in Vial (cells/1ml)   4.7 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   92 Additional information   
Cell No.   JCRB0192 Cell Name   OCUM-1
LOT No.   06252018 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 0.5 mM sodium pyruvate and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.8 - 3.7 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). In some cases, loosely attached cells appear, and they can be hravested by trypsinization.
Doubling Time   apprlx. 34 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   94 Additional information   
Cell No.   JCRB0192 Cell Name   OCUM-1
LOT No.   02092023 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 0.5mM sodium pyruvate and 10% fetal bovine serum (FBS; GIBCO Cat. # 10437). Temperature   37 C
Cell Density at Seeding   1.0 - 5.5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). In some cases, loosely attached cells appear, and they can be hravested by trypsinization.
Doubling Time   approx. 46 hrs. Cell Number in Vial (cells/1ml)   4.0 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   88 Additional information   
Cell No.   JCRB0192 Cell Name   OCUM-1
LOT No.   11292024 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (GIBCO:catalog No.11885-084) with 0.5 mM sodium pyruvate (GIBCO:catalog No.11360-070) and 10% fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K). Temperature   37 C
Cell Density at Seeding   2.2-3.6x10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   83 Antibiotics Used   free
Passage Number   p11* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,10
D13S317:10,11
D7S820:12
D16S539:11
VWA:14,17
TH01:9
AM:X
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   83.1 Additional information   
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