JCRB0193 WB-F344

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0193 Cell Name   WB-F344
Profile   Normal liver epitherial cell line from rat, Fischer-344. Other Name   
Animal   rat Strain   Fischer-344
Genus   Rattus Species   norvegicus
Sex   M Age   adult
Identity    Tissue for Primary Cancer   liver
Case history   normal Metastasis   
Tissue Metastasized    Genetics   Normal diploid cell line. Mode of chromosome number is 40+XY at passage 6.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Normal rat liver epitherial cell line grew in a monolayer. Population doubling was 20-23 hrs at beggining but became 12.5 hrs later. Cells did not accumulate cytoplasmic glycogen, negative for glucose-6-phosphatase.
Classify   normal Established by   Tsao,M-S.
Registered by   Kyoizumi,M. Regulation for Distribution   
Comment    Year   2002
Medium   Eagle's minimal essential medium with 7% fetal calf serum. Methods for Passages   
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_9806
Reference
Pubmed id:11240127Inhibition of gap junctional intercellular communication in rat liver epithelial cells with transforming RNA.
Hayashi T,Trosko JE,Hamada K
FEBS Lett. 2001 Mar 2;491(3):200-6
Pubmed id:9683275Cooperative effects of v-myc and c-Ha-ras oncogenes on gap junctional intercellular communication and tumorigenicity in rat liver epithelial cells.
Hayashi T,Nomata K,Chang CC,Ruch RJ,Trosko JE
Cancer Lett. 1998 Jun 19;128(2):145-54
Pubmed id:9029175Stimulation of cell proliferation and inhibition of gap junctional intercellular communication by linoleic acid.
Hayashi T,Matesic DF,Nomata K,Kang KS,Chang CC,Trosko JE
Cancer Lett. 1997 Jan 15;112(1):103-11
Pubmed id:6468534A diploid epithelial cell line from normal adult rat liver with phenotypic properties of 'oval' cells.
Tsao MS,Smith JD,Nelson KG,Grisham JW
Exp Cell Res. 1984 Sep;154(1):38-52

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LOT Information

Cell No.   JCRB0193 Cell Name   WB-F344
LOT No.   12272014 Lot Specification   distribution
Medium   MEM(GIBCO) with 7% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.3-4.4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.63x10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   p35 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0193 Cell Name   
LOT No.   08072006 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 7% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   84.1 Antibiotics Used   free
Passage Number   P31 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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