JCRB0194 KON

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0194 Cell Name   KON
Profile   Human squamous carcinoma cell line from oral floor. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   76
Identity   available Tissue for Primary Cancer   oral
Case history   squamous cell carcinoma in oral floor, cervical lymph node metastasis observed 6 months after radiation therapy (inside 30Gy, outside 50.4Gy). Metastasis   Yes
Tissue Metastasized   cervical lymph node Genetics   Mode of chromosome=62.5 (53-68).
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Td=32.5 hrs. Colony forming unit = 3%. Expression of proteins at 67, 58 and 54 kDa and keratin were observed. 21 kDa protein from ras P21 was observed.
Classify   tumor Established by   Ohara,M. et al.
Registered by   Kyoizumi,S. Regulation for Distribution   
Comment   Cells were unique by STR-PCR. Year   2002
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum. Methods for Passages   Cells were harvested after treatment with 0.25% trypsin for 15 minutes. Medium change twice a week.
Cell Number on Passage   5 x 10^4 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   cervical lymph node
Tissue Type   squamous cell carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3001
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1298215
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LOT Information

Cell No.   JCRB0194 Cell Name   KON
LOT No.   01262007 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 484735) Temperature   37 C
Cell Density at Seeding   0.5-1.5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA at 37 C for 5-10 min, subculture twice a week.
Doubling Time   43 hrs Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (17 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   Ampule mislabeled as Lot: 012620047. Correct lot# is 01262007.
Cell No.   JCRB0194 Cell Name   KON
LOT No.   03142018 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 25 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS- DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   93 Additional information   
Cell No.   JCRB0194 Cell Name   KON
LOT No.   06162023 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 42 hrs. Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,13
D13S317:11,12
D7S820:8,11
D16S539:9,11
VWA:17
TH01:7,9
AM:X
TPOX:9,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   99 Additional information   Registry # on cryotube (JCRB1094) is mislabeled. Correct registry # is JCRB0194.
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