JCRB1002 K562/ADM
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1002 | Cell Name | K562/ADM |
|---|---|---|---|
| Profile | Adriamycine resistant derivative of K562 cell line. | Other Name | K562/ADM |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 53 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | chronic myelogenous leukemia, adriamycin resistant | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | Chronic myelogenous leukemia cell line, resistant to adriamycin (300ng/ml), induction of p-glycoprotein by addition of sodium butyrate (10ng/ml). |
| Classify | mutant | Established by | Tsuruo, T. |
| Registered by | Tsuruo, T. | Regulation for Distribution | |
| Comment | Mycoplasma decontaminated by Tsuruo at 1987. Multi registancy for antibiotics in cultured cell line derived from human. | Year | 1987 |
| Medium | RPMI1640 medium with 10% fetal calf serum. | Methods for Passages | Dilution. |
| Cell Number on Passage | 2x10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | pleural effusion |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2924298 | Competitive inhibition by verapamil of ATP-dependent high affinity vincristine binding to the plasma membrane of multidrug-resistant K562 cells without calcium ion involvement. Naito M,Tsuruo T Cancer Res. 1989 Mar 15;49(6):1452-5 |
| Pubmed id:3032411 | DNA-mediated transfer and cloning of a human multidrug-resistant gene of adriamycin-resistant myelogenous leukemia K562. Sugimoto Y,Tsuruo T Cancer Res. 1987 May 15;47(10):2620-5 |
| Pubmed id:3091555 | Characteristics of resistance to adriamycin in human myelogenous leukemia K562 resistant to adriamycin and in isolated clones. Tsuruo T,Iida-Saito H,Kawabata H,Oh-hara T,Hamada H,Utakoji T Jpn J Cancer Res. 1986 Jul;77(7):682-92 |
| Pubmed id:6684716 | Effects of inducers of erythroid differentiation of human leukemia K562 cells on vincristine-resistant K562/VCR cells. Okabe-Kado J,Hayashi M,Honma Y,Hozumi M,Tsuruo T Leuk Res. 1983;7(4):481-5 |
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LOT Information
| Cell No. | JCRB1002 | Cell Name | K562/ADM |
|---|---|---|---|
| LOT No. | 02202002 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% heat inactivated fetal calf serum. | Temperature | 37 C |
| Cell Density at Seeding | 1.7 x 10^5 cells/ml | Methods for Passages | Simple dilution. |
| Doubling Time | Td = 22 hrs | Cell Number in Vial (cells/1ml) | 2.0 x 10^6 |
| Viability at cell freezing (%) | 99.0 | Antibiotics Used | free |
| Passage Number | P4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeB), NP, LDH examined. Human, not HeLa. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:8 D7S820:9,11 D16S539:11,12 VWA:16 TH01:10 AM:X TPOX:8,9 CSF1PO:9,10 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1002 | Cell Name | K562/ADM |
|---|---|---|---|
| LOT No. | 01312014 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). | Temperature | 37 C |
| Cell Density at Seeding | 1.3 - 2.5 x 10^5 cells/mL | Methods for Passages | Simple dilution (suspension culture) |
| Doubling Time | Cell Number in Vial (cells/1ml) | 4.2 x 10^6 | |
| Viability at cell freezing (%) | 89.1 | Antibiotics Used | free |
| Passage Number | Unknown (7 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 89.1 | Additional information |