JCRB0006 HL60RG

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0006 Cell Name   HL60RG
Profile   Subline of the HL60 having faster growth rate but the capabilities of the differentiation by TPA or etc were declined. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   36
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   promyelocytic leukemia Metastasis   
Tissue Metastasized    Genetics   DNA prifiling of this cell line is same as HL60.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   HL60 RG grow faster than HL60. Characteristics of the differentiation observed in the HL60 was lost in this cell line.
Classify   variant Established by   Collins,S.J. et.al.
Registered by   Takeuchi,M. Regulation for Distribution   No regulation
Comment   RG:rapid growth. Ability for the differentiation by TPA or DMSO reduced. Year   1985
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution.
Cell Number on Passage   1-2x10^5 cells/ml Race   Caucasian
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2945
Reference
Pubmed id:6192693Fundamentals of chemotherapy of myeloid leukemia by induction of leukemia cell differentiation.
Hozumi M
Adv Cancer Res. 1983;38():121-69
Pubmed id:6932215Induction of differentiation of cultured human promyelocytic leukemia cells by retinoids.
Honma Y,Takenaga K,Kasukabe T,Hozumi M
Biochem Biophys Res Commun. 1980 Jul 31;95(2):507-12
Pubmed id:6930676Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid.
Breitman TR,Selonick SE,Collins SJ
Proc Natl Acad Sci U S A. 1980 May;77(5):2936-40
Pubmed id:271272Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture.
Collins SJ,Gallo RC,Gallagher RE
Nature. 1977 Nov 24;270(5635):347-9

Images



LOT Information

Cell No.   JCRB0006 Cell Name   
LOT No.   12182006 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   dilution, passage twice a week.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0006 Cell Name   HL60RG
LOT No.   082094 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (Bio cell, 4001776) Temperature   37 C
Cell Density at Seeding   5-8x10^5 cells/10cm dish Methods for Passages   Suspend cells by pipetting into fresh medium.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   94.7 Antibiotics Used   free
Passage Number   P12* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Human B(NP,G6PD,PepB,MPI,AST,LD)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0006 Cell Name   HL60RG
LOT No.   F00951 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (Bio Whittaker, lot.3M0581) Temperature   37 C
Cell Density at Seeding   5-8x10^6 cells/10 cm dish Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.0x10^6
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   P18* PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0006 Cell Name   HL60RG
LOT No.   052092 Lot Specification   distribution
Medium   RPMI1640 (lot.043092,lot.050792) + FBS 10% (J.R.S.lot.Do10400) Temperature   37 C
Cell Density at Seeding   1.5x10^6 Methods for Passages   dilution (approx 1/2 dilution)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1x10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   P10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP,LD,G6PD
Chromosome Mode   M=43 Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:8,11
D7S820:11,12
D16S539:11
VWA:16
TH01:8
AM:X
TPOX:8,11
CSF1PO:13,14
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0006 Cell Name   HL60RG
LOT No.   101587 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   1-2x10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   93% Antibiotics Used   free
Passage Number   P9* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0006 Cell Name   HL60RG
LOT No.   07282023 Lot Specification   distribution
Medium   RPMI1640 medium (GIBCO:Cat No.11875) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.03x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.12x10^6
Viability at cell freezing (%)   98 Antibiotics Used   95
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:8,11
D7S820:11,12
D16S539:11
VWA:16
TH01:8
AM:X
TPOX:8,11
CSF1PO:13,14
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   95 Additional information   
Images
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