-Detailed Information [JCRB1003]-

JCRB1003 Kasumi-1

Cell information

Important Notice(s)

On the particles appear in cultures of Kasumi-series cell lines

--- On the small particles appear in cultures of Kasumi-series cell lines ---

(--- Kasumiシリーズ細胞株の培養に現れる微粒子について　---)

During the cultures of Kasumi-series cell lines, self propagating small particles, like microorganisms, appear in the culture medium. These particles were investigated by Iwate University (*1), and were reported that they are consisted mainly of hydroxy apatite and fetuin but are lacking nucleic acids (DNA, RNA). These revealed not to be mycoplasmal, bacteria or yeast conatmination.

(*1) Harasawa,R. et al., In vitro Cell. Dev. Biol. Anim. 42: 13-15, 2006.

At present, it is unknown why these particles increase, and there is no evidence that these are live particles.

Kasumiシリーズ細胞株の培養には微生物様の微細な顆粒が出現して増加します。この顆粒は、岩手大の解析により、リン酸カルシウムとフェチュインを主成分とし、核酸がみられないことが確認されており、マイコプラズマ、細菌、真菌（酵母）ではありません。この粒子がなぜ増えるのかは不明であり、生物であるという証拠はありません。

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Surface antigen expressions in Kasumi-1 cells.

[Surface antigen expressions in Kasumi-1 cells.]

There are some differences in surface antigen expressions between the original report of Kasumi-1 and the FACS analysis by JCRB Cell Bank.

According to the original paper by the developer (Blood 77:2031-2036, 1991), the Kasumi-1 is described as CD13, 33, 34, 38 and HLA-DR positive. However, JCRB's FACS analysis revealed that CD13, 33, 34 and 38 expressions were diminished and the HLA-DR was barely detected. 

The reason why these descripancy occurred, but there is the possibility that the characteristics of Kasumi-1 gradually change after the development of cells to the deposition at Cell Bank.

[FOllowings are written in Japanese.]

Kasumi-1細胞の細胞表面抗原の発現は、原著論文の記述とJCRBが実施したFACS解析で違いが認められました。

細胞樹立者らによる原著論文(Blood 77:2031-2036, 1991)では、Kasumi-1はCD13, 33, 34, 38, HLA-DRは陽性と記述されています。しかし、JCRB細胞バンクが実施したFACS解析では、CD13, 33, 34, 38が低下しており、HLA-DRの発現がほとんど認められませんでした。 

これらの差異がいかなる経緯で生じたのかは不明ですが、細胞樹立時からバンクへの寄託までに徐々に性質が変化した可能性は考えられます。

Kasumi-1細胞の使用に際しましては、原著論文の記述とは上記のような差異がありますことをご承知おきください。

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Cell type：general cells (View Pricing Information)

JCRB No.	JCRB1003	Cell Name	Kasumi-1
Profile	A novel leukemia cell line with an t8;21 chromosome translocation established from a patient attaked by acute myeloid leukemia(AML).	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	7
Identity	available	Tissue for Primary Cancer	hemo-lymphocytic
Case history	aculte myeloid leukemia (AML).	Metastasis
Tissue Metastasized		Genetics	t(8:21), AML-ETO fusion gene
Life Span	infinite	Crisis PDL
Morphology	myeloblast	Character	MPO+, CD34+, CD13+
Classify	tumor	Established by	Asou,H.
Registered by	Kyoizumi,S. &Asou,H.	Regulation for Distribution	free
Comment		Year	2000
Medium	RPMI1640 medium with 10% heat inactivated fetal calf serum.	Methods for Passages	Subculture twice a week at split ratio of 1/2 -1/3 by simple dilution. Suspension cell culture.
Cell Number on Passage	Split ratio = 1/2-1/3.	Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	blood
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0589
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	907275

Reference
Pubmed id:11283671	AML1-ETO downregulates the granulocytic differentiation factor C/EBPalpha in t(8;21) myeloid leukemia. Pabst T,Mueller BU,Harakawa N,Schoch C,Haferlach T,Behre G,Hiddemann W,Zhang DE,Tenen DG Nat Med. 2001 Apr;7(4):444-51
Pubmed id:8334990	The t(8;21) translocation in acute myeloid leukemia results in production of an AML1-MTG8 fusion transcript. Miyoshi H,Kozu T,Shimizu K,Enomoto K,Maseki N,Kaneko Y,Kamada N,Ohki M EMBO J. 1993 Jul;12(7):2715-21
Pubmed id:1720541	t(8;21) breakpoints on chromosome 21 in acute myeloid leukemia are clustered within a limited region of a single gene, AML1. Miyoshi H,Shimizu K,Kozu T,Maseki N,Kaneko Y,Ohki M Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10431-4
Pubmed id:2018839	Establishment of a human acute myeloid leukemia cell line (Kasumi-1) with 8;21 chromosome translocation. Asou H,Tashiro S,Hamamoto K,Otsuji A,Kita K,Kamada N Blood. 1991 May 1;77(9):2031-6

Research results by users. Click！
Pubmed id:32180206	Kasumi leukemia cell lines: characterization of tumor genomes with ethnic origin and scales of genomic alterations. Kasai F,Asou H,Ozawa M,Kobayashi K,Kuramitsu H,Satoh M,Kohara A,Kaneko Y,Kawamura M Hum Cell. 2020 Jul;33(3):868-876

Images

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LOT Information

Cell No.	JCRB1003	Cell Name	Kasumi-1
LOT No.	03232010	Lot Specification	distribution
Medium	RPMI1640 medium with 10% heat-inactivated fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	4 - 10 x 10^4 cells/ml	Methods for Passages	Simple dilution (suspension culture)
Doubling Time	approx. 4-5 days.	Cell Number in Vial (cells/1ml)	7.3 x 10^5
Viability at cell freezing (%)	89.1	Antibiotics Used	free
Passage Number	Unknown (8 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information	On vial: lot number is mis-labeled as 01023230, correctly 03232010.

Cell No.	JCRB1003	Cell Name
LOT No.	02262002	Lot Specification	distribution
Medium	RPMI1640 medium with 10% heat inactivated fetal calf serum. (Use 20% serum at thawing)	Temperature	37 C
Cell Density at Seeding	Split ratio = 1/2-1/3.	Methods for Passages	Subculture twice a week at split ratio of 1/2 -1/3 by simple dilution. Suspension cell culture.
Doubling Time		Cell Number in Vial (cells/1ml)	>10^6
Viability at cell freezing (%)	NT	Antibiotics Used	fee
Passage Number		PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	5% DMSO-Culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1003	Cell Name	Kasumi-1
LOT No.	09032015	Lot Specification	distribution
Medium	RPMI1640 medium with 10% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091).	Temperature	37 C
Cell Density at Seeding	3.1 - 4.9 x 10^5 cells/mL	Methods for Passages	Simple dilution (suspension culture), split ratio of 1/2 -1/3 is recommended.
Doubling Time	approx. 3 days	Cell Number in Vial (cells/1ml)	2.4 x 10^6
Viability at cell freezing (%)	72.2	Antibiotics Used	free
Passage Number	Unknown (8 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% heat-inactivated FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	72.2	Additional information

Cell No.	JCRB1003	Cell Name	Kasumi-1
LOT No.	02092018	Lot Specification	distribution
Medium	RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396)	Temperature	37 C
Cell Density at Seeding	2.8-5.2x10^5 cells/sq.cm	Methods for Passages	dilution
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.0x10^6
Viability at cell freezing (%)	97.40	Antibiotics Used	free
Passage Number	p8*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	NT	Additional information	Start culture under 2 ml growth medium,because cell number is under 1.0x10^6 cells/vial.

Images

Cell No.	JCRB1003	Cell Name	Kasumi-1
LOT No.	05112018	Lot Specification	distribution
Medium	RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396)	Temperature	37 C
Cell Density at Seeding	3.0-4.3x10^5 cells/sq.cm	Methods for Passages	dilution
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.8x10^6
Viability at cell freezing (%)	97.10	Antibiotics Used	free
Passage Number	p8*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:9,11 D13S317:11,13 D7S820:8,11 D16S539:9,12 VWA:14 TH01:6,9 AM:X TPOX:8,9 CSF1PO:10,12
Adhesion	No	Exoteric Gene	NT
Medium for Freezing	BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	80	Additional information

Images

Cell No.	JCRB1003	Cell Name	Kasumi-1
LOT No.	07252022	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437)	Temperature	37 C
Cell Density at Seeding	approx. 6 x 10^5 cells/mL	Methods for Passages	Simple dilution (suspension culture)
Doubling Time	54 - 95 hrs.	Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	80.2	Antibiotics Used	free
Passage Number	Unknown (8 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:9,11 D13S317:11,13 D7S820:8,11 D16S539:9,12 VWA:14 TH01:6,9 AM:X TPOX:8,9 CSF1PO:10,12
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	80.0	Additional information