JCRB1004 Kasumi-3

Cell information

Important Notice(s)

On the particles appear in cultures of Kasumi-series cell lines

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1004 Cell Name   Kasumi-3
Profile   A novel human leukemia cell line established from blast cells of a patient attaked by myeloperoxidase-negative acute leukemia. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   57
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   myeloperoxidase-negative acute leukemia Metastasis   
Tissue Metastasized    Genetics   t(3:7)
Life Span   infinite Crisis PDL   
Morphology   myeloblast Character   Overexpression of Evi-1 protein, CD34+, CD7+
Classify   tumor Established by   Asou,H.
Registered by   Kyoizumi,S. &Asou,H. Regulation for Distribution   free
Comment    Year   2000
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum. Methods for Passages   Subculture twice a week at split ratio of 1/2 by simple dilution. Suspension cell culture.
Cell Number on Passage   Split ratio=1/2 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0612
Reference
Pubmed id:10482986Activation of EVI1 transcripts with chromosomal translocation joining the TCRVbeta locus and the EVI1 gene in human acute undifferentiated leukemia cell line (Kasumi-3) with a complex translocation of der(3)t(3;7;8).
Suzukawa K,Kodera T,Shimizu S,Nagasawa T,Asou H,Kamada N,Taniwaki M,Yokota J,Morishita K
Leukemia. 1999 Sep;13(9):1359-66
Pubmed id:8613429Establishment of an undifferentiated leukemia cell line (Kasumi-3) with t(3;7)(q27;q22) and activation of the EVI1 gene.
Asou H,Suzukawa K,Kita K,Nakase K,Ueda H,Morishita K,Kamada N
Jpn J Cancer Res. 1996 Mar;87(3):269-74


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Pubmed id:32180206 Kasumi leukemia cell lines: characterization of tumor genomes with ethnic origin and scales of genomic alterations.
Kasai F,Asou H,Ozawa M,Kobayashi K,Kuramitsu H,Satoh M,Kohara A,Kaneko Y,Kawamura M
Hum Cell. 2020 Jul;33(3):868-876

Images



LOT Information

Cell No.   JCRB1004 Cell Name   
LOT No.   11172010 Lot Specification   seed/1st distribution
Medium   RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   3 x 10^5 cells/ml Methods for Passages   Simple dilution, split ratio = 1:2 is recommended.
Doubling Time   Slow (>4 days) Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   81.5 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1004 Cell Name   
LOT No.   12032010 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   3 - 4 x 10^5 cells/ml Methods for Passages   Simple dilution, split ratio = 1:2 is recommended.
Doubling Time   Slow (> 4 days) Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   87.9 Antibiotics Used   free
Passage Number   Unknown (18 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No. Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   Yes
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1004 Cell Name   Kasumi-3
LOT No.   02282002 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:11,12
D7S820:10,11
D16S539:9,11
VWA:16,18
TH01:6,9
AM:XY
TPOX:8
CSF1PO:11
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1004 Cell Name   Kasumi-3
LOT No.   04272016 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   1.2 - 4.5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). Subculture twice a week at split ratio of 1/2.
Doubling Time   Slow (more than 4 days) Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   72.0 Antibiotics Used   free
Passage Number   unknown (19 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   72.0 Additional information   
Cell No.   JCRB1004 Cell Name   Kasumi-3
LOT No.   06172022 Lot Specification   distribution
Medium   RPMI 1640 medium with 20% FBS (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   Slow (above 4 days) Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P31* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:11,12
D7S820:10,11
D16S539:9,11
VWA:16,18
TH01:6,9
AM:X,Y
TPOX:8
CSF1PO:11
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   67 Additional information   
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