JCRB1005 NCC-IT-A3
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1005 | Cell Name | NCC-IT-A3 |
|---|---|---|---|
| Profile | Human teratocarcinoma cell line from anterior mediastinum. | Other Name | NCCIT |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 24 |
| Identity | available | Tissue for Primary Cancer | teratocarcinoma |
| Case history | teratocarcinoma | Metastasis | |
| Tissue Metastasized | Genetics | Cells are expressing HST-1 oncogene | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | teratocarcinoma from anterior mediastinum |
| Classify | tumor | Established by | Teshima, S. |
| Registered by | Teshima, S. | Regulation for Distribution | |
| Comment | Cells were obtained from tissue and were cloned later by the dilution method. | Year | 1990 |
| Medium | RPMI1640 medium with 10% fetal calf serum. | Methods for Passages | Mechanical pipetting is enough, which was informed originally. |
| Cell Number on Passage | split ratio=1/5 - 1/10 | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | anterior mediastinum |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:3060118 | Differential expression of two homologous and clustered oncogenes, Hst1 and Int-2, during differentiation of F9 cells. Yoshida T,Muramatsu H,Muramatsu T,Sakamoto H,Katoh O,Sugimura T,Terada M Biochem Biophys Res Commun. 1988 Dec 15;157(2):618-25 |
| Pubmed id:2974287 | Two homologous oncogenes, HST1 and INT2, are closely located in human genome. Wada A,Sakamoto H,Katoh O,Yoshida T,Yokota J,Little PF,Sugimura T,Terada M Biochem Biophys Res Commun. 1988 Dec 15;157(2):828-35 |
| Pubmed id:3178812 | Expression of the HST1 oncogene in human germ cell tumors. Yoshida T,Tsutsumi M,Sakamoto H,Miyagawa K,Teshima S,Sugimura T,Terada M Biochem Biophys Res Commun. 1988 Sep 30;155(3):1324-9 |
| Pubmed id:2895649 | Cloned hst gene from normal human leukocyte DNA transforms NIH3T3 cells. Sakamoto H,Yoshida T,Nakakuki M,Odagiri H,Miyagawa K,Sugimura T,Terada M Biochem Biophys Res Commun. 1988 Mar 30;151(3):965-72 |
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LOT Information
| Cell No. | JCRB1005 | Cell Name | |
|---|---|---|---|
| LOT No. | 01192004 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (FBS Lot; GIBCO 3762782S) | Temperature | 37 C |
| Cell Density at Seeding | 6 - 7 x 10^4 cells/ml | Methods for Passages | Pipetting, or 0.25% tryspin and 0.02% EDTA. The former was recommended by depositor, but apparently the latter method resulted good recovery. |
| Doubling Time | 27 hrs (max) | Cell Number in Vial (cells/1ml) | 3.3 x 10^6 |
| Viability at cell freezing (%) | 91.7 | Antibiotics Used | free |
| Passage Number | P15* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1005 | Cell Name | NCC-IT-A3 |
|---|---|---|---|
| LOT No. | 07192002 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal calf serum (Intergen RB52405). | Temperature | 37 C |
| Cell Density at Seeding | 1.2 x 10^4 cells/sq.cm. | Methods for Passages | Cells harvested by piptting. Cells are quite weak when vigorous handling is done when subculture. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.9 x 10^6 |
| Viability at cell freezing (%) | 82.5 | Antibiotics Used | free |
| Passage Number | P14* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeB), NP, LDH examined. Human, not HeLa. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,13 D13S317:11 D7S820:10 D16S539:9,12 VWA:14,18 TH01:7,9 AM:X TPOX:8 CSF1PO:10,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture mediium containing 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1005 | Cell Name | NCC-IT-A3 |
|---|---|---|---|
| LOT No. | 11162017 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | approx. 7 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 28 hrs. | Cell Number in Vial (cells/1ml) | 1.8 x 10^6 |
| Viability at cell freezing (%) | 92.1 | Antibiotics Used | free |
| Passage Number | Unknown (15 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 92.1 | Additional information |