JCRB1006.0 OUMS-36

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1006.0 Cell Name   OUMS-36
Profile   Normal human embryo fibroblast cell. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   embryo
Identity   available Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   normal cell
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   normal fibroblast
Classify   normal Established by   Kouchi,Y. & Namba,M.
Registered by   Namba,M. Regulation for Distribution   
Comment    Year   2002
Medium   Delbecco's modified Eagle's medium with 10% fetal bovine serum. Methods for Passages   Cells harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3091
Reference
Pubmed id:noneNormal huma fibroblast cell lines tranfected with the hTRT gene -OUMS36T cell line series-
Kouchi,H. and Namba,M.
Tiss. Cult. Res. Commun., 19: 203-204, 2000.

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LOT Information

Cell No.   JCRB1006.0 Cell Name   OUMS-36
LOT No.   04222002 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum(RB52405). Temperature   37 C
Cell Density at Seeding   2.7 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.1% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   97.1 Antibiotics Used   BM-cycline and MC210 only for three weeks.
Passage Number   P26 (+12PDL) PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(type B), NP and LDH. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,14,15
D13S317:8
D7S820:12,13
D16S539:10,11
VWA:18
TH01:8,9.3
AM:X
TPOX:8
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1006.0 Cell Name   OUMS-36
LOT No.   04232002 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum(RB52405). Temperature   37 C
Cell Density at Seeding   2.7 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.1% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   97.5 Antibiotics Used   BM-cycline and MC210 only for three weeks.
Passage Number   P26 (+12PDL) PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(type B), NP and LDH. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:8,11
D7S820:11
D16S539:9,10
VWA:18,20
TH01:6,9
AM:X
TPOX:8
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1006.0 Cell Name   OUMS-36
LOT No.   05082002 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   2.5 x 10^3 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.1% trypsin. Subculture once a week and medium change twice a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   97.3 Antibiotics Used   BM-cycline and MC210 treatment only for 3 weeks.
Passage Number   P28(+16PDL) PDL   +16PD
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(type B), NP and LDH. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1006.0 Cell Name   OUMS-36
LOT No.   07042013 Lot Specification   distribution
Medium   Delbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. #10099). Temperature   37C
Cell Density at Seeding   4 - 8 x 10^4 cells/mL Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.7 x 10^6 (cells/1m
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P28 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   91.2% Additional information   
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