JCRB1010 KMRC-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1010 Cell Name   KMRC-1
Profile   Human tumor cell line from kidney. Clear cell renal carcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   adult
Identity   available Tissue for Primary Cancer   kidney
Case history   brain metastasis was developed after radical rephrectomy Metastasis   
Tissue Metastasized    Genetics   VHL gene alteration observed.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Clear cell renal carcinoma cell line
Classify   tumor Established by   Ashida,S. et.al.
Registered by   Shuin,T. Regulation for Distribution   not applied
Comment   P16 at deposit. Year   2001
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. Subculture once every 4-5 days.
Cell Number on Passage   5.0 x 10^3 cells/sq.cm. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2983
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1298168
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LOT Information

Cell No.   JCRB1010 Cell Name   
LOT No.   04212005 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   2 - 4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1.2 days Cell Number in Vial (cells/1ml)   6.4 x 10^5
Viability at cell freezing (%)   86.1 Antibiotics Used   free
Passage Number   P27 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1010 Cell Name   KMRC-1
LOT No.   08112002 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   5.3 x 10^3 cells/cm^2 Methods for Passages   Cells harvested after treatment with 0.25% trypsin. Subculture once a week, Metium change twice a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   96.6 Antibiotics Used   free
Passage Number   P23 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1010 Cell Name   KMRC-1
LOT No.   07152014 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.4 - 5.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   8.4 x 10^5
Viability at cell freezing (%)   94.4 Antibiotics Used   free
Passage Number   P26 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11
D13S317:8
D7S820:11,12
D16S539:9,12
VWA:17,19
TH01:7,8
AM:X
TPOX:8
CSF1PO:9,10
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   94.4 Additional information   
Cell No.   JCRB1010 Cell Name   KMRC-1
LOT No.   06082018 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.4 - 3.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 29 hrs. Cell Number in Vial (cells/1ml)   9.0 x 10^5
Viability at cell freezing (%)   92.7 Antibiotics Used   free
Passage Number   P27 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   92.7 Additional information   
Cell No.   JCRB1010 Cell Name   KMRC-1
LOT No.   04042022 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   1.0 - 5.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 41 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   P27 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   97.9 Additional information   
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