JCRB0008 CKT-1 Cell information
Cell type:general cells
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JCRB No.
JCRB0008
Cell Name
CKT-1
Profile
useful to assay bovine adenovirus 3
Other Name
Animal
bovine
Strain
Genus
Bos
Species
taurus
Sex
M
Age
Identity
Tissue for Primary Cancer
kidney
Case history
Metastasis
Tissue Metastasized
Genetics
kidney
Life Span
infinite
Crisis PDL
Morphology
Character
fibroblast-like kidney epithelial cell
Classify
Established by
Kimura,Y.
Registered by
Takeuchi,M.
Regulation for Distribution
Not defined.
Comment
Year
1985
Medium
Eagle's minimal essential medium with 10% fetal calf serum.
Methods for Passages
Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage
split ratio=1/10
Race
CO2 Conc.
5 %
Tissue Sampling
kidney
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Reference
Pubmed id:4303468 A new cell line of calf kidney and its susceptibility to viruses. Kimura S,Fukui K,Yoshida N,Matsubara Y Jpn J Microbiol. 1968 Sep;12(3):293-8
LOT Information
Cell No.
JCRB0008
Cell Name
CKT-1
LOT No.
071089
Lot Specification
distribution
Medium
MEM 90% + FCS 10%
Temperature
37
Cell Density at Seeding
1/10 dilution
Methods for Passages
0.02% EDTA and 0.25% Trypsin.
Doubling Time
NT
Cell Number in Vial (cells/1ml)
4.1x10^6
Viability at cell freezing (%)
NT
Antibiotics Used
free
Passage Number
P11
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
Chromosome Information
NT
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information